摘要
目的探讨B亚型血清学鉴定及基因测序分析。方法采用血型血清学方法鉴定ABO血型,采用PCR-SSP、ABO基因分型、基因测序等方法进行分型和序列分析。结果血清学鉴定发现4例B亚型,其中基因型B303/O2 3例、Bw11/02 1例;基因测序发现第3内含子intron3+5 G/A杂合3例、nt695 T/C杂合突变1例。结论采用血型血清学鉴定,结合基因分型技术是正确鉴定ABO亚型的策略。
Objective To explore the serological identification and gene sequencing analysis of subtype B. Methods Serological method is performed to identify the ABO blood group, and PCR-SSP, ABO genotyping and gene sequencing are carried out to analyze the genotypes and sequences. Results Four cases of subtype B were found by serological identification, the genotype of which included 3 cases of B303/O2 and 1 case of Bw11/02. 3 cases of intron3+5 G/A heterozygote and 1 case of nt695 T/C heterozygote mutation were revealed by gene sequencing. Conclusion The combination of genotyping and serological identification is the correct strategy for identifying ABO subtypes.
作者
胡思群
朱碎永
施顺秋
钟锦灶
林甲进
HU Si-qun;ZHU Sui-yong;SHI Shun-qiu;ZHONG Jin-zao;LIN Jia-jin(The Second Affiliated Hospital&Yuying Childrens Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325027,China;不详)
出处
《中国卫生检验杂志》
CAS
2019年第23期2837-2839,共3页
Chinese Journal of Health Laboratory Technology
基金
温州市科技计划项目(Y20170209)
贺林院士新医学临床转化工作站科研基金重点项目(18331104)
平阳县社会发展科技计划项目(Y2018A35)
关键词
B亚型
血清学
基因序列分析
B subtype
Serological
Gene sequence analysis
