摘要
研究旨在探究饲料中高剂量精氨酸对虹鳟性能,摄食后不同时段虹鳟血浆和肝脏中游离精氨酸、鸟氨酸、瓜氨酸及尿素的水平,肠道精氨酸酶Ⅱ(Arg2)、诱导一氧化氮合酶(iNOS)以及热休克蛋白(HSP70)基因表达的影响。将不同组别虹鳟分别饲喂含0(对照组)、2%(T1)、4%(T2)晶体精氨酸的基础饲料,饲养周期为64 d。结果显示:相比对照组,T1组具有较高的蛋白质沉积率(P<0.05)。监测摄食后血浆游离氨基酸水平发现,T2组血浆精氨酸含量持续增长到摄食后12 h;血浆精氨酸水平仅在试验组显著升高(P<0.05)。另外,在摄食后18 h,对照组血浆瓜氨酸水平显著高于T2组;对照组肝脏瓜氨酸水平显著高于T2组(P<0.05);T2组血浆和肝脏中游离鸟氨酸水平明显高于对照组,而试验组肠道Arg2和HSP70表达量均显著高于对照组(P<0.05)。结果表明:血浆中瓜氨酸含量可间接反映内源性精氨酸合成水平,肠道精氨酸酶Ⅱ可能参与调节高水平精氨酸的代谢机制。
The study was conducted to investigate the effect of excessive arginine supplementation on growth performance,arginine,ornithine,citrulline and urea levels of hepatic and plasma at postprandial different times,expression of arginase II(Arg2),inducible nitric oxide synthase(iNOS),and heat shock protein 70(HSP70)genes in the intestine.Rainbow trout were fed diets containing 1.47%(control group),3.89%(T1 group)and 5.64%(T2 group)arginine for 64 days.The results showed that higher muscle protein content was observed in T1 group than control group(P<0.05).In moitor postprandial amino acid level study observed:plasma arginine of T2 group kept increased until 12 h.Significant increase of plasma arginine level was only observed in arginine supplemented group(P<0.05).Meanwhile,plasma citrulline level in CTRL was significantly higher than in T2 group at 18 h.A significantly higher hepatic citrulline level was also observed in control group than in T2 group(P<0.05).Significantly higher plasma and hepatic free ornithine level was observed in T2 group than control group while significantly higher expression of intestinal Arg2 and HSP70 was found in arginine supplemented groups(P<0.05).The above results indicate that:the content of citrulline in plasma can indirectly reflect the level of endogenous arginine synthesis,intestinal arginase Ⅱ may participate in regulating the high level of arginine metabolism mechanism.
出处
《饲料研究》
CAS
北大核心
2019年第11期50-56,共7页
Feed Research
基金
山东省现代农业产业技术体系生猪创新团队生产与环境控制岗位项目(项目编号：SDAIT-08-09)
关键词
氨基酸水平
精氨酸
生长性能
虹鳟
基因表达
尿素水平
amino acid level
arginine
growth performance
rainbow trout
gene expression
urea level
