摘要
目的建立网果酸模Rumex chalepensis中6个成分的含量测定方法。方法采用HPLC法,色谱柱Agilent Extend-C18(250 mm×4.6 mm,5μm),254 nm波长处二极管阵列检测器检测,流动相为甲醇-0.1%甲酸,梯度洗脱,体积流量1 mL/min,柱温25℃,进样量5μL。结果对照品大黄酚-8-O-β-D-葡萄糖苷、大黄素-8-β-D-吡喃葡萄糖苷、酸模素、大黄素、大黄酚、大黄素甲醚分别在208~3 120、22.40~336.35、178.9~2 908.8、16.7~250.8、104.4~1 566.0、45.2~677.7 ng,线性关系良好,平均回收率(n=6)分别为97.66%、97.10%、98.78%、97.38%、102.48%和95.51%。16批网果酸模中大黄酚-8-O-β-D-葡萄糖苷、大黄素-8-β-D-吡喃葡萄糖苷、酸模素、大黄素、大黄酚、大黄素甲醚的质量分数分别为0.6~7.1、0~2.7、1.0~6.5、0.1~0.6、0.7~4.3、0.1~0.4 mg/g;对比分析不同生长年限、不同采收期、不同地块样品含量,2年生网果酸模初春或夏末初秋采集,6个成分含量总和较高,为12.2mg/g。结论所建立的方法可用于同时测定网果酸模中6个成分含量,确定了网果酸模的采收年限和季节,为网果酸模药材质量评价标准的制定提供了科学依据。
Objective To establish a method for the determination of six components from Rumex chalepensis Mill..Methods The contents of chrysophanol-8-O-β-D-glucoside,emodin-8-β-D-glucoside,nepodin,emodin,chrysophanol,and physcion were simultaneously determined by HPLC.The mobile phase was methanol-0.1%formic acid,gradient elution,flow rate of 1 mL/min,column temperature of 25℃,injection volume of 5μL,detected by Agilent Extend-C18(250 mm×4.6 mm,5μm)and diode array detector at 254 nm wavelength.Results The content of chrysophanol-8-O-β-D-glucoside,emodin-8-β-D-glucoside,nepodin,emodin,chrysophanol,and physcionhad good linear relationship in the ranges of 208—3120,22.40—336.35,178.9—2908.8,16.7—250.8,104.4—1566.0,45.2—677.7 ng,respectively.The average recovery rates were 97.66%,97.10%,98.78%,97.38%,102.48%,and 95.51%(n=6).The contents of chrysophanol-8-O-β-D-glucoside,emodin-8-β-D-glucoside,nepodin,emodin,chrysophanol,and physcion in 16 batches of R.chalepensis.were determined in the range of 0.6—7.1,0—2.7,1.0—6.5,0.1—0.6,0.7—4.3,and 0.1—0.4 mg/g,respectively.Sample contents of different growing years,harvesting dates,and plots were compared and analyzed.Two-year-old R.chalepensis.was collected in early spring or late summer and early autumn.The total content of six components was 12.2 mg/g,which was relatively high.Conclusion The established method can be used for simultaneous determination of six components from R.chalepensis,and determine the harvesting time and season of R.chalepensis,which provides a scientific basis for the formulation of quality evaluation criteria of R.chalepensis.
作者
郭昌洪
许京
王瑞海
苗青
严光俊
杨诗龙
刘丽梅
GUO Chang-hong;XU Jing;WANG Rui-hai;MIAO qing;YAN Guang-jun;YANG Shi-long;LIU Li-mei(Institute of Basic Theory Research of Traditonal Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;Hubei Jingzhou Hospital of Traditional Chinese Medicine,Jingzhou 434000,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第21期5342-5345,共4页
Chinese Traditional and Herbal Drugs
基金
中国中医科学院基本科研业务费自主选题项目(YZ-1721)
湖北省卫生健康委员会2018—2019年度中医药科研立项项目(鄂卫通[2019] 13号-ZY2019F015)
荆州市2018年度科技发展计划项目(荆科技发[2018]37号-2018091)
