摘要
试验旨在探究广西某地区猪源大肠杆菌的耐药性及部分菌株所含耐药基因情况,为预防及治疗动物性大肠杆菌类疾病提供参考。采用麦康凯琼脂培养基及伊红美蓝琼脂培养基初步鉴定、显微镜镜检、分子生物学16S rRNA鉴定、药物敏感性二倍肉汤微量稀释法等方法进行大肠杆菌的分离鉴定,并结合PCR相关技术对部分菌株的三十余种耐药基因进行检测。结果显示,从广西某地区采集的猪源病料中共分离得到65株猪源大肠杆菌,其均为多重耐药大肠杆菌且多集中于六重耐药与七重耐药。按所耐抗菌药物的种类分析发现其对β-内酰胺类、磺胺类、大环内酯类药物具有较高耐药性,耐药率分别98.46%、90.77%及84.62%,进一步分析发现其主要是对头孢噻呋钠、阿莫西林、磺胺间甲氧嘧啶、阿奇霉素等抗菌药物具有较高耐药性,对美罗培南、阿米卡星、黏杆菌素较为敏感。对部分菌株所含耐药基因进行整理发现,其多含有四环素类(tetA、tetM)、β-内酰胺类(CTX-m-u)及磺胺类(Sul2)耐药基因。综上所述发现广西地区猪源大肠杆菌耐药性情况较为严重,所含耐药基因也相对较多,因此在实际生产中需采用联合用药等相关措施进行疾病治疗以达到快速高效治疗疾病的目的。
The aim of this study was to analyze the drug resistance of porcine Escherichia coli in some areas of Guangxi and drug resistance genes contained in some strains,and provide a reference for the prevention and treatment of animal Escherichia coli diseases.The experiment used Macconkey agar medium and Eosin-Methylene Blue agar medium preliminary identification,microscopic microscopy,molecular biology 16S rRNA identification,drug sensitivity double broth microdilution method and other methods to isolate and identify Escherichia coli,combined with PCR-related techniques were used to detect more than 30 drug resistance genes in some strains.The results showed that 65 strains of porcine Escherichia coli were isolated from pig-derived materials collected from a certain area of Guangxi,all of which were multi-drug resistant Escherichia coli and concentrated in six-drug resistance and seven-drug resistance.According to the investigation of the antibiotic-resistant drugs,it was found to be highly resistant toβ-lactams,sulfonamides,macrolides,and the drug resistance rates were 98.46%,90.77%and 84.62%,respectively.Further analysis results found that it was mainly resistant to antibiotics such as ceftiofur sodium,amoxicillin,sulfamonomethoxine,azithromycin,and sensitive to meropenem,amikacin and colistin.The resistance genes contained in some strains were found to contain tetracycline(tetA and tetM),β-lactams(CTX-m-u)and sulfonamide(Sul2)resistance genes.In summary,it was found that the drug resistance of porcine Escherichia coli in Guangxi was serious,and there were relatively many drug resistance genes.Therefore,in the actual production,it was necessary to use related measures such as combination therapy for disease treatment to achieve rapid and effective treatment of diseases.
作者
李崇
陈韵如
李沁媚
孙俊颖
安朝霞
覃凤林
陈心瑜
沈水宝
司红彬
LI Chong;CHEN Yunru;LI Qinmei;SUN Junying;AN Zhaoxia;QIN Fenglin;CHEN Xinyu;SHEN Shuibao;SI Hongbin(College of Animal Science and Technology,Guangxi University,Nanning 530005,China)
出处
《中国畜牧兽医》
CAS
北大核心
2019年第12期3749-3758,共10页
China Animal Husbandry & Veterinary Medicine
基金
广西科技重大专项(桂科AA17204057)
贵港市科技成果转化及应用(贵科转1829008)
国家自然科学基金项目(31760746)
广西大学“大学生创新创业训练计划”(201810593293)
关键词
猪源大肠杆菌
抗菌药物
耐药性
耐药基因
porcine Escherichia coli
antibacterial drugs
drug resistance
drug resistance gene
