摘要
目的探讨血清长链非编码RNA(lncRNA)MALAT1和AFAP1-AS1与鼻咽癌临床病理特征和预后的关系。方法2013年4月—2015年6月在我院经病理证实的鼻咽癌患者136例为鼻咽癌组,同期选择我院门诊健康体检者54例为对照组,实时荧光逆转录法分析MALAT1和AFAP1-AS1的表达;采用χ2检验分析MALAT1和AFAP1-AS1表达与临床病理特征的关系;Log-rank检验分析血清MALAT1和AFAP1-AS1不同表达水平患者的预后差异。结果与对照组比较,鼻咽癌组MALAT1和AFAP1-AS1表达水平明显升高,差异有统计学意义(P<0.001);MALAT1和AFAP1-AS1表达与年龄无关(P>0.05);肿瘤最大径≥5 cm、病理学分期越高、TNM分期越高、浸润深度越深、有淋巴血管间隙浸润、有淋巴结转移、有复发的鼻咽癌患者MALAT1和AFAP1-AS1高表达率升高(P<0.05);MALAT1和AFAP1-AS1低表达组2年生存率及生存期均明显高于MALAT1和AFAP1-AS1高表达组(P<0.001);多因素Cox逐步回归分析,结果发现MALAT1低表达(HR=0.52,95%CI0.37~0.81)和AFAP1-AS1低表达(HR=0.56,95%CI0.51~0.83)为鼻咽癌患者预后的独立保护因素(P<0.001)。结论鼻咽癌患者血清lncRNA MALAT1、AFAP1-AS1水平升高,且与鼻咽癌恶性进展有关;鼻咽癌患者血清MALAT1、AFAP1-AS1低表达患者预后良好;MALAT1、AFAP1-AS1可能作为诊断鼻咽癌的新型标志物。
Objective The objective of this study was to investigate the relationship between serum long-chain non-coding RNA MALAT1 and AFAP1-AS1 and clinicopathological features,and prognosis of nasopharyngeal carcinoma(NPC).Methods A total of 136 patients with nasopharyngeal carcinoma confirmed by pathology in our hospital were selected from April 2013 to June 2015.During the same time,54 outpatients for health examination in our hospital were selected as the control group and nasopharyngeal carcinoma group.Real-time fluorescence reverse transcription analysis was used to analyze the expression of lncRNA MALAT1 and AFP1-AS1.The relationship between the expression of lncRNA MALAT1 and AFP1-AS1,and clinicopathological characteristics was analyzed byχ2 test.Log-rank assay was used to analyze serum long-chain non-coding RNA MALAT1 and prognostic differences in patients with different expression levels of AFAP1-AS1.Results Compared with the control group,the serum levels of RNA MALAT1 and RNA AFAP1-AS1 in the nasopharyngeal carcinoma group were significantly increased(P<0.001);The expressions of lncRNA MALAT1 and AFAP1-AS1 was not related to age(P>0.05);The maximum diameter of the tumor was≥5 cm,the pathological stage was higher,the TNM stage was higher,the deeper in the infiltration depth,the infiltration of lymphatic vessels,the lymph node metastasis,and the recurrence and the higher in high expressive rates of lncRNA MALAT1 and AFAP1-AS1(P<0.05).The 2-year survival rate and survival time of lncRNA MALAT1 and AFAP1-AS1 in the low expression group were significantly higher than those of the high expression group(P<0.001);Multivariate Cox stepwise regression analysis showed that low expression of lncRNA MALAT1(HR=0.52,95%CI0.37~0.81)and low expression of lncRNA AFAP1-AS1(HR=0.56,95%CI0.51~0.83)were independent prognostic protective factors for NPC patients(P<0.001).Conclusion The serum long-chain non-coding RNA MALAT1 and AFAP1-AS1 are elevated in patients with NPC,and are positively correlated with malignant progression of NPC.NPC patients with low expression of lncRNA MALAT1 and AFAP1-AS1 serum have a good prognosis;MALAT1 and AFAP1-AS1 can be used as new markers for the diagnosis of nasopharyngeal carcinoma.
作者
刘耿淳
申良方
LIU Gengchun;SHEN Liangfang(Department of Oncology,Xiangya Hospital Central South University,Changsha 410008,China)
出处
《实用肿瘤学杂志》
CAS
2019年第6期513-518,共6页
Practical Oncology Journal
基金
2014年度湖南省医药卫生科研计划(编号:C2014-49)
