摘要
本研究旨在通过比较7个品种甘薯叶提取物的抗氧化活性和α-葡萄糖苷酶抑制活性,探讨甘薯叶抗氧化和降血糖的潜力。以甘薯叶为研究对象,测定其总多酚和总黄酮含量,体外实验评价其抗氧化活性(DPPH·清除能力、ABTS^+·清除能力、·OH清除能力和还原能力)及对α-葡萄糖苷酶的抑制活性。结果表明7个品种甘薯叶提取物总多酚和总黄酮含量、抗氧化活性以及α-葡萄糖苷酶抑制活性具有明显差异。甘薯叶总多酚含量介于15.02~33.33 mg GAE/g DW,总黄酮含量介于1.06~3.11 mg RE/g DW。徐薯18、济薯18和龙薯9甘薯叶总多酚和总黄酮含量最高,品种间无统计学差异(P> 0.05)。龙薯9自由基(DPPH·、ABTS^+·、·OH)清除能力最强;济薯18还原能力最强;商薯19抑制α-葡萄糖苷酶活性最强。总多酚和总黄酮含量与抗氧化活性之间具有极显著的正相关性(P <0.01),而与α-葡萄糖苷酶抑制活性之间没有显著的相关性(P> 0.05)。本研究表明甘薯叶是潜在抗氧化剂和α-葡萄糖苷酶抑制剂的优质来源,为甘薯种植和生产品种的选择提供理论依据。
This study aims to investigate the antioxidant and hypoglycemic potentials of sweet potato( Ipomoea batatas L.) leaves by comparing antioxidant activity and α-glucosidase inhibitory activity of sweet potato leaf extracts from 7 varieties.The content of total polyphenols and flavonoids in sweet potato leaves was measured. The antioxidant activity( DPPH· scavenging ability,ABTS^+·scavenging ability,·OH scavenging and reducing ability) and α-glucosidase inhibitory activity were evaluated by in vitro experiments.The results showed that there were significant differences in the content of total polyphenols and flavonoids,antioxidant activity and α-glucosidase inhibitory activity among sweet potato leaf extracts from 7 varieties. The content of total polyphenols in sweet potato leaves ranged from 15.02 to 33.33 mg GAE/g DW,and the content of total flavonoids from 1.06 to 3.11 mg RE/g DW. The content of total polyphenols and flavonoids was the highest in sweet potato leaves of Xushu 18,Jishu 18 and Longshu 9,without statistical differences( P > 0.05). Free radical scavenging ability( DPPH·,ABTS^+·,·OH) was the highest in Longshu 9. The reducing ability was the highest in Jishu 18. Shangshu 19 presented the highest α-glucosidase inhibitory activity.The content of total polyphenols and flavonoids showed an extremely positive correlation with antioxidant activity( P < 0.01),but no obvious correlation with α-glucosidase inhibitory activity( P > 0.05). This study demonstrates that sweet potato leaves are a good source of potential antioxidants and α-glucosidasee inhibitors,providing a theoretical basis for sweet potato cultivation and the selection of sweet potato varieties.
作者
刘冉
程霜
王雷
曾庆华
张书贤
LIU Ran;CHENG Shuang;WANG Lei;ZENG Qing-hua;ZHANG Shu-xian(Agricultural College,Liaocheng University,Liaocheng 252000,China)
出处
《食品工业科技》
CAS
北大核心
2019年第23期283-289,共7页
Science and Technology of Food Industry
基金
博士启动基金项目(318051603)
关键词
甘薯叶
多酚
抗氧化
Α-葡萄糖苷酶
品种
黄酮
sweet potato leaf
polyphenols
antioxidant
α-glucosidase
variety
flavonoids
