摘要
目的:研究藏药传统方三味豆蔻汤对小鼠睡眠的改善作用。方法:将180只SPF级昆明种小鼠随机分为空白组、对照组、三味豆蔻汤高剂量组、三味豆蔻汤剂低量组、牛奶混合物高剂量组、牛奶混合物低剂量,每组10只。空白组以生理盐水、对照组以牛奶、低剂量组以1.56 g/kg/d(人12g/d)、高剂量组以3.12 g/kg/d(人24g/d)对小鼠连续灌胃给药30天,于末次灌胃后分别进行直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验和巴比妥钠睡眠潜伏期实验,比较不同给药组与空白组之间的差异。结果:三味豆蔻汤、牛奶混合物以及牛奶均无直接诱导睡眠的作用,且三味豆蔻汤高剂量、牛奶混合物低剂量对延长小鼠睡眠时间和缩短睡眠潜伏期具有显著性作用(P<0.05);牛奶混合物高剂量则具有极显著作用(P<0.01),明显延长了小鼠睡眠时间和缩短睡眠潜伏期。结论:三味豆蔻汤具有一定的助睡眠作用,且与牛奶联合使用,可能起协同助睡眠功效。
The effect of traditional Tibetan medicine SanWeiDouKou decoction on sleep in mice was studied with 180 SPF Kunming mice.180 SPF Kunming mice were randomly divided into 6 groups with 10 mice in each group.The divided groups are such as blank group treated with physiological salt solution,control group treated with milk,high dose of SanWeiDouKou decoction group treated with 3.12 g/kg/d(human 24g/d)SanWeiDouKou decoction,low dose of SanWeiDouKou decoction group treated with 1.56 g/kg/d(human 12g/d)SanWeiDouKou decoction,high dose of SanWeiDouKou decoction mixing with milk group treated with 3.12 g/kg/d(human 24g/d)SanWeiDouKou decoction mixing with milk and low dose of SanWeiDouKou decoction mixing with milk group treated with 1.56 g/kg/d(human 12g/d)SanWeiDouKou decoction mixing with milk,and treaded for 30 days.The direct sleep experiment,pentobarbital sodium sleep prolongation experiment,pentobarbital sodium subliminal dose hypnosis experiment and barbiturate sodium sleep latency experiment were conducted respectively after the last gavage.The differences between different dosing groups and the blank group were analyzed.There was no direct effect of SanWeiDouKou decoction,SanWeiDouKou decoction mixing with milk and milk on the induction of sleep.However,the high dose of SanWeiDouKou decoction and low dose of SanWeiDouKou decoction mixing milk have significant effects on prolonging sleeping time and shortening sleep latency of mice(P<0.05).In particularl,the high doses of SanWeiDouKou decoction mixing milk have a significant effect on prolonging sleep time and shortening sleep latency in mice(P<0.01).In conclusion,SanWeiDouKou decoction has a certain sleep-aid effect,and combined with milk,it may have a synergistic sleep-aid effect.
作者
卓玛东智
秀措吉
袁瑞瑛
巴珠
Zhuoma-Dongzhi;XIU Cuoji;YUAN Ruiying;Ba Zhu(Medical College of Tibet University,Lhasa 850000,China;Key Laboratory of Tibetan Medicine Moder Research in Tibet Autonomous Region,Lhasa 850000,China;Jiri Community Health Service Center,Chengguan District,Lhasa 850000,China;Clinical laboratory of Tibetan hospital,Lhasa 850000,China)
出处
《高原科学研究》
CSCD
2019年第4期73-77,共5页
Plateau Science Research
基金
西藏自治区自然科学基金（XZ2018ZRG-09）
西藏自治区藏医药管理局基金（JJKT2018008)
关键词
藏药
三味豆蔻汤
睡眠功能
Tibetan medicine
SanWeiDouKou decoction
sleeping-improvement function
