摘要
目的探究慢性阻塞性肺疾病(COPD)不同阶段Th1/Th2细胞因子与免疫炎症的关系。方法纳入我院2017年8月至2019年7月间COPD急性加重(AECOPD)患者30例、COPD缓解期患者30例以及健康人群对照组30例,采用酶联免疫吸附法检测外周血趋化因子受体3(CXCR3)、γ-干扰素(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)和白细胞介素-13(IL-13)水平,采用固相酶联免疫斑点法检测弹性蛋白作为抗原刺激CD4^+ T淋巴细胞分泌IFN-γ水平。结果与对照组相比,AECOPD组和COPD缓解组CXCR3水平更高(F=11.43,P<0.001)。与COPD缓解组相比,AECOPD组IFN-γ水平更低,且两组IFN-γ水平均高于对照组(F=13.62,P<0.001)。AECOPD组和缓解组IL-2、IL-4、IL-13高于对照组(F分别为10.25、10.36、8.58,P<0.001)。30 ng/mL弹性蛋白作为抗原刺激的CD4^+Th1细胞分泌的IFN-γ多于15 ng/mL弹性蛋白。结论 COPD患者中CXCR3高表达,AECOPD大量Th1细胞参与免疫炎症被消耗。CD4^+ Th1激活可能是COPD免疫炎症反应的原因。
Objective To explore the relationship between Th1/Th2 cytokines and immune inflammation in different stages of chronic obstructive pulmonary disease(COPD).Methods From August,2017 to July,2019,30 patients with acute exacerbation of COPD(AECOPD),30 patients with remission of COPD and 30 healthy individuals were enrolled for the study.Chemokine receptor 3(CXCR3),interferon-gamma(IFN-γ),interleukin-2(IL-2),interleukin-4(IL-4)and interleukin-13(IL-13)in peripheral blood were detected by ELISA.Different concentrations of elastin were used as antigens to stimulate the secretion of IFN-γ from CD4^+ T lymphocytes by ELISPOT.Results Compared with the control group,CXCR3 levels in the AECOPD group and COPD remission group were higher(F=11.43,P<0.001).Compared with the COPD remission group,the level of IFN-γ in the AECOPD group was lower,and the levels of IFN-γ in two groups were higher than that in the control group(F=13.62,P<0.001).The levels of IL-2,IL-4 and IL-13 in the AECOPD group and remission group were higher than those in the control group(F values:10.25,10.36 and 8.58 respectively,P<0.001).The IFN-γ secreted by CD4^+Th1 cells stimulated by 30 ng/mL elastin was more than 15 ng/mL elastin.Conclusion CXCR3 is highly expressed in COPD patients,and AECOPD depletes a large number of Th1 cells involved in immune inflammation.Activation of CD4^+ Th1 may be the cause of COPD immune inflammation.
作者
陈俊
李乾兵
徐裕丰
CHEN Jun;LI Qian-bing;XU Yu-feng(Respiratory Medicine Department,The First People’s Hospital of Anqing,Anqing 246000,China)
出处
《标记免疫分析与临床》
CAS
2019年第11期1863-1866,1875,共5页
Labeled Immunoassays and Clinical Medicine
