摘要
目的探讨阿加曲班对急性脑梗死大鼠神经功能损伤后的影响及可能机制。方法将60只SD大鼠随机分为4组,包括假手术组、模型组(改良Longa线栓法造模)、阿加曲班+模型组(简称阿加曲班组)、安慰剂+模型组(简称安慰剂组)。假手术组采用的操作步骤与模型组相同,但不插入线栓。从大鼠术后第一天开始,腹腔注射阿加曲班3 mg/kg,连续给药7 d,安慰剂组给予等体积的生理盐水。术后第8天,Zea-Longa评分检测大鼠神经功能,TTC染色检测大鼠脑梗死体积,Western blot检测Cx43蛋白和Klotho蛋白的表达。结果模型组Zea-Longa评分明显高于假手术组(P<0.05),阿加曲班组评分与模型组比较显著降低(P<0.05),安慰剂组评分与模型组比较差异无统计学意义(P>0.05)。模型组脑梗死面积明显大于假手术组(P<0.05),阿加曲班组脑梗死面积与模型组比较明显缩少(P<0.01),安慰剂组脑梗死面积与模型组比较差异无统计学意义(P>0.05)。模型组Cx43蛋白的表达显著高于假手术组(P<0.05),阿加曲班组Cx43蛋白的表达与模型组比较明显降低(P<0.05),安慰剂组Cx43蛋白的表达与模型组比较差异无统计学意义(P>0.05)。模型组klotho蛋白的表达明显低于假手术组(P<0.05),阿加曲班组klotho蛋白的表达与模型组比较明显增加(P<0.05),安慰剂组klotho蛋白的表达与模型组比较差异无统计学意义(P>0.05)。结论阿加曲班对急性脑梗死大鼠神经功能损伤有明显的改善作用,其机制可能涉及Kotho蛋白。
Objective To investigate the effect of argatroban on neurological injury in rats with acute cerebral infarction and its potential mechanism.Methods Sixty SD rats were randomly divided into 4 groups,including the sham operation group,model group(modified Longa line plug method),argatroban + model group(abbreviated as argatroban group),and the placebo + model group(abbreviated as placebo group).The sham operation group used the same procedure as the model group,but was not inserted a thread plug.From the first day after the operation on the rats,argatroban of 3 mg/kg was intraperitoneally administered for 7 days,and the placebo group was given an equal volume of physiological saline.On the 8 th day after the operation,the Zea-Longa score was used to detect the neurological function of the rats.The infarct volume was detected by TTC staining,and the expressions of Cx43 and Klotho protein were detected by western blot.Results The Zea-Longa score of the model group was significantly higher than that of the sham operation group(P<0.05) and the score of the argatroban group was significantly lower than that of the model group(P<0.05).There was no significant difference between the placebo group and the model group(P>0.05).The cerebral infarct size of the model group was significantly larger than that of the sham operation group(P<0.05).The cerebral infarction area of the argatroban group was significantly less than that of the model group(P<0.01),while there was no significant difference between the placebo group and the model group(P>0.05).The expression of Cx43 protein in the model group was significantly higher than that in the sham operation group(P<0.05).The expression of Cx43 protein in the argatroban group was significantly lower than that in the model group(P<0.05).The expression of Cx43 protein in the placebo group was not different from the model group(P>0.05).The expression of klotho protein in the model group was significantly lower than that in the sham operation group(P<0.05).The expression of klotho protein in the argatroban group was significantly increased compared with the model group(P<0.05).The expression of klotho protein in the placebo group was not different from the model group(P>0.05).Conclusion Argatroban has a significant improvement effect in neurological impairment in rats with acute cerebral infarction,and its mechanism may involve Kotho protein.
作者
刘娟
尹航
刘猛
朱月敏
LIU Juan;YIN Hang;LIU Meng;ZHU Yue-min(Department of Neurology,Zhuozhou City Hospital,Zhuozhou 072750,China)
出处
《标记免疫分析与临床》
CAS
2019年第11期1935-1939,共5页
Labeled Immunoassays and Clinical Medicine
基金
保定市社发类项目(编号:18zf1532)
