摘要
从石油烃污染土壤中分离得到菌株L-2,对石油烃(TPH)的降解进行研究。通过16S rRNA鉴定L-2为肠杆菌属。采用基于Box-Behnken设计(BBD)的响应面方法 (RSM),考察温度、pH和TPH质量浓度3个自变量对TPH降解率的交互效应,建立二次回归方程预测模型,确定3个实验因素的最佳组合,通过方差分析验证该二次模型的有效性。确定系数R2=0.9943,表明二次模型与实验数据吻合较好。响应面优化得到最佳降解条件:温度为30℃,pH为7.13和TPH质量浓度为3.93g/L,TPH降解率为87.79%。结果表明,温度对TPH降解速率的影响(P<0.0001)较其他两个参数更显著。GC/MS结果表明菌株L-2可降解十六烷(C16)至二十五烷(C25)范围内的TPH组分。
The degradation of total petroleum hydrocarbon(TPH) was studied by using L-2 strains isolated from oil-contaminated soil. L-2 was identified as Enterobacter sp. by 16 S rRNA. Response surface method(RSM) based on box-behnken design(BBD) was adopted to evaluate the interaction effects of temperature, pH and TPH concentration on TPH degradation. A quadratic regression model was established to determine the best combination of the three experimental factors and the validity of the model was verified by analysis of variance(ANOVA). The value of determination coefficient(R2=0.9943)indicated a satisfactory agreement between the quadratic model and the experimental data. In the temperature of 30℃, pH of 7.13 and TPH concentration of 3.93 g/L, the best degradation rate was 87.79%.It was found that TPH degradation rate was more significantly affected(p<0.0001) by temperature compared with other two parameters. GC/MS results indicated that TPH components in the range of hexadecane(C16) to pentadecane(C25) can be degraded ty strain L-2.
作者
孙烁
刘其友
陈水泉
赵朝成
于文赫
SUN Shuo;LIU Qiyou;CHEN Shuiquan;ZHAO Chaocheng;YU Wenhe(College of Chemical Engineering,China University of Petroleum(East China),Qingdao 266580,Shandong,China)
出处
《化工进展》
EI
CAS
CSCD
北大核心
2019年第12期5512-5518,共7页
Chemical Industry and Engineering Progress
基金
石油石化污染物控制与处理国家重点实验室开放课题(PPC2018014)
山东省自然科学基金(ZR2014BM023)
关键词
肠杆菌属
生物修复
石油烃
优化
气相色谱/质谱
Enterobacter sp.
bioremediation
total petroleum hydrocarbon(TPH)
optimization
GC/MS
