摘要
目的观察到1例疣状毛癣菌(Trichophyton verrucosum,T.verrucosum)致须癣患者的局部皮损中存在大量中性粒细胞。以往文献报道中性粒细胞通过形成胞外诱捕网(Neutrophils extracellular traps, NETs)参与抗真菌免疫,但T.verrucosum能否诱导NETs形成,及NETs是否具有抗T.verrucosum作用尚不清楚,故探究中性粒细胞是否通过形成NETs参与杀灭T.verrucosum。方法收集患者病历资料。从患者组织分离、鉴定T.verrucosum。免疫荧光法检测患者及T.verrucosum感染小鼠组织中的NETs。T.verrucosum刺激正常人外周血中性粒细胞,免疫荧光法检测NETs的形成。NETs与T.verrucosum共孵育,提取T.verrucosum DNA,通过实时荧光定量PCR定量检测真菌数量,观察NETs对T.verrucosum的杀伤作用。结果 T.verrucosum感染患者及小鼠组织可见明显NETs。T.verrucosum与中性粒细胞共孵育后有明显NETs形成。与NETs孵育后T.verrucosum存活率低于对照组(P<0.01)。结论 T.verrucosum可以诱导中性粒细胞产生NETs,NETs对T.verrucosum具有显著杀伤作用。
Objective A large number of neutrophils were found in the local lesions of patient with tinea barbae caused by Trichophyton verrucosum(T. verrucosum). Previous literatures reported that neutrophils participate in antifungal immunity through the formation of extracellular traps(NETs), but it was unclear whether T. verrucosum could induce NETs formation and whether NETs were involved in anti-T. verrucosum, so we explored whether neutrophils participate in the killing of T. verrucosum by forming NETs. Methods The patient’s clinical data was collected. T. verrucosum was isolated and identified from patient’s tissues. NETs in the tissues of patients and T. verrucosum-infected mice were observed by immunofluorescence. T. verrucosum was used to stimulate peripheral neutrophils in normal subjects, immunofluorescence was used to detect the formation of NETs. NETs were incubated with T. verrucosum, and then T. verrucosum DNA was extracted, Real-time quantitative PCR was used to quantitatively detect the killing effect of NETs on T. verrucosum. Results NETs formation could be seen in the tissues of T. verrucosum infected patients and mice. T. verrucosum co-incubation with neutrophils showed NETs formation. The survival rate of T. verrucosum after incubation with NETs was lower than that of the control group(P<0.01). Conclusions T. verrucosum can induce neutrophils to produce NETs, and NETs have a significant killing effect on T. verrucosum.
作者
张秀军
郭艳阳
朱振来
薛小文
马翠玲
王刚
付萌
ZHANG Xiu-jun;GUO Yan-yang;ZHU Zhen-lai;XUE Xiao-wen;MA Cui-ling;WANG Gang;FU Meng(Department of Dermatology at Xijing Hospital,the Fourth Military Medical University,Xi’an,Shaanxi 710032,China)
出处
《中国真菌学杂志》
CSCD
2019年第6期326-331,共6页
Chinese Journal of Mycology
