摘要
目的探究miRNA-424、miRNA-375、miRNA-34a、miRNA-92a、miRNA-93联合经阴道彩色多普勒超声(TVCDS)诊断宫颈癌的临床价值。方法选择术后经病理证实为宫颈癌患者133例(宫颈癌组)、宫颈上皮瘤变患者130例(CIN组)及子宫良性病变患者130例(对照组)。分析比较三组miRNA-424、miRNA-375、miRNA-34a、miRNA-92a、miRNA-93的表达水平,超声血流参数及这些指标的诊断灵敏度、特异度。结果宫颈癌组miRNA-424、miRNA-375、miRNA-34a表达量明显低于CIN组及对照组,miRNA-92a、miRNA-93表达量明显高于CIN组及对照组,差异均有统计学意义(q分别=26.06、22.60、9.96、4.97、8.41;55.62、68.53、44.67、18.42、13.21,P均<0.05);宫颈癌组宫颈前后径、左右径明显大于CIN组与对照组,差异均有统计学意义(q分别=17.18、14.80、23.41,17.57,P均<0.05),而收缩期峰值血流速度(PSV)明显快于CIN组与对照组,阻力指数(RI)明显低于CIN组与对照组,差异有统计学意义(q分别=23.23、8.36、39.66、40.89,P均<0.05);血清miRNA联合TVCDS诊断宫颈癌灵敏度、特异度明显高于血清miRNA-424、miRNA-375、miRNA-34a、miRNA-92a、miRNA-93、TVCDS,差异均有统计学意义(χ2分别=11.35、9.78、9.03、7.62、8.32、8.52;14.14、12.22、6.00、5.08、4.32、168.07,P均<0.05)。结论血清miRNA联合TVCDS诊断宫颈癌具有无创、灵敏度高、特异度高等优势,对宫颈癌疾病的诊断及预后判断均具有指导价值。
Objective To investigate the clinical value of miRNA-424,miRNA-375,miRNA-34a,miRNA-92a,and miRNA-93 combined with transvaginal color doppler ultrasound(TVCDS)in the diagnosis of cervical cancer(CC).Methods Totally 133 patients with cervical cancer were enrolled as CC group,130 patients with cervical epithelial neoplasia were enrolled as CIN group,and 130 patients with benign uterine lesions were selected as normal group.The expression levels of miRNA-424,miRNA-375,miRNA-34a,miRNA-92a and miRNA-93 in three groups were analyzed and compared,as well as ultrasonic blood flow parameters,diagnostic sensitivity and specificity.Results The expression levels of miRNA-424,miRNA-375 and miRNA-34a in CC group were significantly lower than those in CIN group and normal group,and the expression levels of miRNA-92a and miRNA-93 were significantly higher than those in CIN group and normal group(q=26.06,22.60,9.96,4.97,8.41;55.62,68.53,44.67,18.42,13.21,P<0.05).The anteroposterior diameter and the left to right cervical diameter of CC group were significantly larger than those of CIN group and normal group,and the differences were statistically significant(q=17.18,14.80,23.41,17.57,P<0.05).The PSV of CC group was significantly faster than CIN group and normal group,the RI of CC group was significantly lower than CIN group and normal group(q=23.23,8.36,39.66,40.89,P<0.05).The specificity and sensitivity of serum miRNA combined with TVCDS in the diagnosis of CC were significantly higher than those of miRNA-424,miRNA-375,miRNA-34a,miRNA-92a,miRNA-93,and TVCDS(χ2=11.35,9.78,9.03,7.62,8.32,8.52;14.14,12.22,6.00,5.08,4.32,168.07,P<0.05).Conclusion The combination of serum miRNA with TVCDS in the diagnosis of CC has the advantages of non-invasive,high sensitivity and high specificity,which has guiding value for the diagnosis and prognosis of CC disease.
作者
叶玲红
陈方红
姚奏英
陈志辉
吕晴
杨惠芬
YE Linghong;CHEN Fanghong;YAO Zouying(Department of Ultrasound,Lishui Central Hospital,Lishui323000,China)
出处
《全科医学临床与教育》
2019年第12期1068-1071,F0002,共5页
Clinical Education of General Practice
基金
浙江省医药卫生科技计划项目(2017KY730)
