摘要
为筛选出可产抑菌肽的芽孢菌,采用稀释涂布、平板划线的方法从土壤、泡菜、湖泥等10种样品中分离纯化菌种,利用抑菌圈法筛选出有4株抑菌性的芽孢菌,对抑菌效果最好的菌株J11通过菌落形态、革兰氏染色、芽孢染色、生理生化试验以及16SrDNA测序鉴定,表明其为多粘类芽孢杆菌,并进一步测定J11的抑菌谱,结果表明其对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、蜡样芽孢杆菌、溶酪巨型球菌、戊糖片球菌、瑞士乳酸杆菌、乳酸链球菌均具有一定的抑菌性。尤其对溶酪巨型球菌的抑菌圈直径达30.13 mm,抑菌作用非常明显。J11具有良好的抑菌性,具有广谱抑菌效果,对常见的致病菌和腐败菌都有一定的抑菌性,在食品保藏中具有一定的应用价值。
In order to screen Bacillus strain producing bacteriocin,four Bacillus strains were isolated and purified from soil,pickles,lake mud and other samples by dilution coating and plate marking.Antibacterial bacteria were screened by the method of bacteriostasis circle.The bacterial strains were identified by colony morphology,gram staining,spore staining,physiological and biochemical experiments and 16SrDNA sequencing.Finally,the bacteriostasis circle was used to determine the bacteriostasis circle size of the strains against various indicator bacteria and the bacteriostasis spectrum was obtained.The best bacteriostasis strain J11 was identified as Bacillus polymyxa.J11 was tested for Enterobacter,Staphylococcus aureus,Bacillus subtilis,Bacillus cereus,Caseinolyticus megacoccus,Pentosacoccus,Lactobacillus Switzerland and Streptococcus lactis.The bacteria had certain bacteriostasis.The diameter of bacteriostasis circle for Megacoccus casei was 30.13 mm,which was extremely sensitive to bacteriostasis.J11 had good bacteriostasis and broad-spectrum bacteriostasis effect,which had certain bacteriostasis to common pathogenic bacteria and spoilage bacteria,and had certain application value in food preservation.
作者
满文曾
胡容
封嗣中
严铭玺
张雷春雨
杨焕
周涛
MAN Wen-zeng;HU Rong;FENG Si-zhong(Department of Food Science and Engineering,Nanjing Normal University,Nanjing,Jiangsu 210097)
出处
《安徽农业科学》
CAS
2020年第1期173-175,185,共4页
Journal of Anhui Agricultural Sciences
基金
江苏省农业科技自主创新资金项目[CX(16)1057]
南京师范大学国家级大学生创新项目(201810319030Z)
关键词
筛选
细菌素
芽孢菌
抑菌性
Screen
Bacteriocin
Bacillus
Antibacterial activities
