摘要
制备了十六烷基三甲基溴化铵(CTAB)包被的硒化铜纳米晶体(Cu2-xSe NCs),并对其高效抗菌活性机制进行了研究。革兰氏阴性细菌大肠杆菌(Escherichia coli,E.coli)和革兰氏阳性细菌金黄色葡萄球菌(Staphylococcus aureus,S.aurues)被用作模型菌株,通过探究其最小抑菌浓度(MIC)和最小杀菌浓度(MBC),以及杀菌动力学评估了Cu2-xSe NCs的抗菌性能。实验结果显示,金黄色葡萄球菌对Cu2-xSe NCs更为敏感,这是由于大肠杆菌具有双层膜而金黄色葡萄球菌仅拥有单层膜。此外,当Cu2-xSe NCs浓度为32μg/mL时,不管是革兰氏阳性菌还是革兰氏阴性菌,都会在1 h内全部死亡,证明Cu2-xSe NCs拥有较强的杀菌性能。
Bacteria are the pathogen of many diseases and pose a serious threat to people’s health.Therefore,it is important to develop new antibacterial agents.We prepared a highly effective antibacterial agent,copper selenide nanocrystals(Cu2-xSe NCs),and their coating agent was cetyltrimethylammonium bromide(CTAB).Gram-negative bacteria Escherichia coli(E.coli) and Gram-positive bacteria Staphylococcus aureus(S.aurues) were used as model strains,the antibacterial properties of Cu2-xSe NCs were evaluated by investigating their minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) and bactericidal kinetics.The experimental results show that S.aurues is more sensitive to Cu2-xSe NCs,because E.coli has a double membrane and S.aurues has only a single membrane.In addition,when the concentration of Cu2-xSe NCs is 32 μg/mL,both Gram-positive and Gram-negative bacteria will die in one hour,which proves that Cu2-xSe NCs has strong bactericidal properties and has the potential to become a new antibacterial agent.
作者
吕文毅
李雪
郭青娟
邹鸿雁
黄承志
LüWen-yi;LI Xue;GUO Qing-juan;ZOU Hong-yan;HUANG Cheng-zhi(Key Laboratory of Luminescent and Real-Time Analytical Chemistry(Southwest University),Ministry of Education,College of Pharmaceutical Sciences,Southwest University,Chongqing 400715;Chongqing Key Laboratory of Biomedical Analysis(Southwest University),Chongqing Science&Technology Commission,College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715)
出处
《分析科学学报》
CAS
CSCD
北大核心
2019年第6期783-789,共7页
Journal of Analytical Science
基金
国家自然科学基金(No.21976144)
关键词
硒化铜纳米晶体
抗菌剂
高效抗菌
Copper selenide nanocrystals
Antibacterial agent
Highly effective antibacterial
