摘要
目的采用超声辅助响应面法优化油茶叶总酚的提取工艺。方法以油茶叶多酚含量为指标,在单因素实验基础上运用Box-Behnken响应面法设计四因素三水平提取实验。结果最佳工艺条件为乙醇浓度50%,振幅比30%,超声时间20 min,料液比1:45(m:V),在此条件下测得油茶叶多酚含量为(89.506±0.273) mg/g,与模型预测数值相差较小。结论在最优条件下,油茶叶多酚的提取率得到了提高。
Objective To optimize the ultrasonic assisted extraction of total phenol from oil tea leaves by response surface methodology. Methods With polyphenol content of oil tea leaves as index,the Box-Behnken response surface method was used to design the four-factor and three-level extraction experiments based on the single-factor experiment. Results The best process conditions were 50% ethanol concentration,30% amplitude ratio,ultrasonic time 20 min,and material-liquid ratio 1:45(m:V). Under these conditions,the polyphenol content of Camellia oleifera was(89.506±0.273) mg/g,which has small difference from model predictions. Conclusion Under the optimal conditions,the extraction rate of polyphenols from oil tea leaves is improved.
作者
胡平
吴菲菲
李化强
刘庆
王美英
彭洁
HU Ping;WU Fei-Fei;LI Hua-Qiang;LIU Qing;WANG Mei-Ying;PENG Jie(Department of Food and Chemical Engineering,Shaoyang University,Shaoyang 422000,China)
出处
《食品安全质量检测学报》
CAS
2019年第24期8316-8322,共7页
Journal of Food Safety and Quality
基金
湖南省教育厅科学研究优秀青年项目(17B241)
湖南省教育厅科学研究一般项目(17C1433)
湖南省自然科学基金青年项目(2017JJ3284)
湖南省科技厅“三区”科技人才项目(S2017NCTPSQ0044)
邵阳学院研究生科研创新项目(CX2019SY032、CX2019SY037、CX2019SY040、CX2019SY044)~~
关键词
响应面
油茶叶
总酚
超声提取
response surface analysis
Camellia oleifera leaves
total phenols
ultrasonic extraction
