摘要
目的用,并探讨其可能的机制。方法将SD大鼠分为假手术组(Sham),模型组(LPS)和DMED治疗组(LPS+DMED);称重并计算各组大鼠肺系数、肺组织湿/干重比(W/D)和含水量;HE染色检测各组大鼠肺组织病理变化;ELISA检测各组大鼠肺组织IL-1β、IL-6及TNF-α蛋白的表达;免疫荧光化学法染色各组大鼠肺组织NF-κB(p65)的表达;Western blot法检测TLR9和NF-κB(p65)的蛋白表达。结果与LPS组比较,DMED明显降低感染性休克大鼠的肺系数、肺组织湿/干重比(W/D)和含水量,改善感染性休克大鼠肺组织病理变化,显著降低IL-1β、IL-6及TNF-α蛋白表达水平,减弱肺组织中NF-κB(p65)的活化,抑制TLR9和NF-κB(p65)的蛋白表达。结论 DMED对感染性休克大鼠急性肺损伤的保护作用,与减轻炎症因子和抑制TLR-9/NF-κB信号通路相关。
Objective To investigate the protective effect of dexmedetomidine on acute lung injury and the potential mechanism in septic shock rats. Methods SD rats were randomly divided into:Sham group, model group(LPS treatment), DMED group(treated with DMED). The radio between the lung wet weight and lung dry weight(W/D), pulmonary coefficient and pulmonary moisture capacity were calculated. HE staining was used to observe the pathological changes of lung tissue, the levels of IL-1β, IL-6 and TNF-α proteins were tested by ELISA, the expression of NF-κB(p65) was determined by immunofluesence, the expression of TLR9 and NF-κB(p65) was observed by Western blot. Results Compared with LPS group, DMED reduced the radio between the lung wet weight and dry weight(W/D), pulmonary coefficient and pulmonary moisture capacity, improved the pathological changes of lung tissue, decreased the levels of IL-1β, IL-6 and TNF-α proteins, decreased the activation of NF-κB(p65), inhibited the expression level of TLR9 and NF-κB(p65). Conclusion The therapeutic effect of DMED on acute lung injury is related to inhibitingTLR-9/NF-κB pathway in septic shock rats.
作者
董丽敏
周南
孙莹杰
DONG Li-min;ZHOU Nan;SUN Ying-jie(Department of Anesthesiology,The Second and Third Hospital of Chinese People’s Liberation Army,Heilongjiang Qiqihar 161000;Department of Anesthesiology,Northern Theater,Liaoning 110016,China)
出处
《解剖科学进展》
2019年第6期626-629,633,共5页
Progress of Anatomical Sciences
基金
辽宁省自然科学基金(201602790)
