摘要
目的 探讨血管新生性青光眼(NVG)患者房水中促红细胞生成素(EPO)、血管内皮生长因子(VEGF)检测表达及其与眼血流动力学及氧化应激状况的相关性,为NVG的诊治提供依据。方法 选择2017年1月至2018年12月在中国人民解放军陆军特色医学中心(下称该院)门诊收治的血管新生性青光眼患者35例为研究对象,将其设置为研究组;另选择同期该院收治的原发性青光眼患者35例为对照组。采用酶联免疫法检测两组患者房水中EPO及VEGF表达水平,同时使用超声检测患者眼血流动力学参数及氧化应激状况,并分析其相关性。结果 研究组EPO及VEGF水平分别为(192.34±10.27)ng/mL、(1 089.14±52.18)U/L,显著高于对照组的(49.13±5.48)ng/mL、(305.26±20.58)U/L,差异有统计学意义(P<0.05);研究组视网膜中央动脉EDV、PSA水平及腱状后短动脉EDV、PSA水平分别为(2.51±0.32)、(8.12±1.08)、(2.64±0.41)、(9.74±1.17)cm/s,显著低于对照组的(3.84±0.47)、(12.54±2.16)、(3.75±0.52)、(15.53±2.06)cm/s,差异有统计学意义(P<0.05);研究组患者房水中EPO及VEGF表达水平与视网膜中央动脉EDV、PSA水平及腱状后短动脉EDV、PSA水平存在显著的负相关(r=-0.374,P=0.000);研究组房水中MDA、AOPP水平分别为(3.48±0.49)、(2.51±0.58)nmol/mL显著高于对照组的(1.86±0.18)、(1.09±0.16)nmol/mL,差异有统计学意义(P<0.05);研究组房水中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)水平分别为(21.36±3.46)、(15.97±3.15)、(9.14±2.83)U/mL显著低于对照组的(59.15±8.09)、(42.12±6.76)、(24.12±4.14)U/mL,差异有统计学意义(P<0.05);研究组患者房水中EPO及VEGF表达水平与MDA、AOPP水平呈正相关(r=0.671,P=0.000),与SOD、GSH-Px、CAT水平呈负相关(r=-0.592,P=0.000)。结论NVG患者房水中EPO及VEGF表达水平显著高于原发性青光眼患者,且与眼血流动力学及氧化应激状况相关。检测EPO及VEGF在临床中的发病机制能够助于临床诊断及治疗参考。
Objective To investigate the correlation between erythropoietin(EPO)and vascular endothelial growth factor(VEGF)in aqueous humor of patients with angiogenetic glaucoma(NVG)and their correlation with ocular hemodynamics and oxidative stress,so as to provide evidence for the diagnosis and treatment of NVG.Methods 35 cases of angiogenetic glaucoma admitted to Chinese People′s Liberation Army Characteristic Medical Center from January 2017 to December 2018 were selected as the study group,and 35 cases patients with primary glaucoma admitted to the hospital during the same period were selected as the control group.The expressions of EPO and VEGF in aqueous humor of the two groups were detected by ELISA,and the ocular hemodynamic parameters and oxidative stress were measured by ultrasound,and the correlation was analyzed.Results The levels of EPO and VEGF in the study group were(192.34±10.27)ng/mL and(1089.14±52.18)U/L,respectively,which were significantly higher than those in the control group(49.13±5.48)ng/mL and(305.26±20.58)U/L,with significant differences(P<0.05).The levels of EDV,PSA in the central retinal artery and the posterior tendinous short artery in the study group were(2.51±0.32),(8.12±1.08),(2.64±0.41),(9.74±1.17)cm/s,respectively,which were significantly lower than those in the control group(3.84±0.47),(12.54±2.16),(3.75±0.52),(15.53±2.06)cm/s,with significant differences(P<0.05).The expressions of EPO and VEGF in aqueous humor were negatively correlated with the levels of EDV and PSA in central retinal artery and EDV and PSA in posterior tendinous short artery in the study group(r=-0.374,P=0.000).The levels of MDA and AOPP in aqueous humor of the study group were(3.48±0.49),(2.51±0.58)nmol/mL,which were significantly higher than those of the control group(1.86±0.18),(1.09±0.16)nmol/mL,and the differences were statistically significant(P<0.05).The levels of SOD,GSH-Px and CAT in aqueous humor of the study group were(21.36±3.46),(15.97±3.15),(9.14±2.83)U/mL,which were significantly lower than those of the control group(59.15±8.09),(42.12±6.76),(24.12±4.14)U/mL,and the differences were statistically significant(P<0.05).The expression levels of EPO and VEGF in aqueous humor were positively correlated with the levels of MDA and AOPP(r=0.671,P=0.000),and negatively correlated with the levels of SOD,GSH-Px and CAT(r=-0.592,P=0.000).Conclusion The expression levels of EPO and VEGF in aqueous humor of NVG patients were significantly higher than those of primary glaucoma patients,and were correlated with ocular hemodynamics and oxidative stress.Detection of EPO and VEGF in clinical pathogenesis can be helpful for clinical diagnosis and treatment.
作者
许多
阳建政
刘莛
刘华
朗敏
贺翔鸽
XU Duo;YANG Jianzheng;LIU Yi;LIU Hua;LANG min;HE Xiangge(Department of Ophthalmology,Chinese People′s Liberation Army Characteristic Medical Center,Chongqing 400042,China;Department of Ultrasound,Chinese People′s Liberation Army Characteristic Medical Center,Chongqing 400042,China;Department of Ophthalmology,Chongqing Aer Ophthalmology Hospital,Chongqing 400000,China)
出处
《国际检验医学杂志》
CAS
2020年第1期78-81,86,共5页
International Journal of Laboratory Medicine
