摘要
目的探讨重组人干扰素-α2b(Recombinant human interferon alpha 2b, rhIFN-α2b)联合5-氨基酮戊酸(5-Aminolevulinic acid,ALA)-光动力疗法(photodynamic therapy,PDT)治疗对慢性宫颈炎合并持续人乳头瘤病毒(Human Papillomavirus,HPV)感染患者血清转化生长因子β1(transforming growth factor-β1,TGF-β1)与白细胞介素10(Interleukin-10,IL-10)的影响。方法感染HPV的慢性宫颈炎患者102例,按照数字随机表法分为观察组和对照组,每组患者51例。对照组采用rhIFN-α2b治疗;观察组采用rhIFN-α2b联合ALA-PDT治疗,PDT治疗功率密度80 mW/cm^2,照射时间20~60 min,能量密度150 J/cm^2,共治疗3次,每次治疗间隔7 d。采用qRT-PCR法分别对治疗前、治疗后1和3个月的HPV-DNA负荷量进行检查,并分析治疗后3个月的转阴率;采用酶联免疫法在治疗后1个月分别对两组患者血清中TGF-β1、IL-10蛋白水平进行检测。结果治疗后1和3个月观察组患者HPV-DNA负荷量低于对照组(P<0. 05);观察组转阴率(80. 4%)高于对照组(58. 8%)(P<0. 05);观察组患者血清中TGF-β1、IL-10水平均明显低于对照组,差异具有统计学意义(P<0. 05)。结论 rhIFN-α2b联合ALA-PDT能降低慢性宫颈炎合并持续HPV感染可降低患者血清中TGF-β1、IL-10水平,可减少宫颈癌发生率的风险。
Objective To investigate the combined effect of recombinant human interferon alpha 2 b and Ira-photodynamic therapy on serum transforming growth factor beta 1 and interleukin 10 in patients with chronic cervicitis complicated with persistent human papillomavirus infection.Methods Totally 102 patients of chronic cervicitis infected with HR-HPV were divided evenly into two groups with digital random table:the observation group(51 patients)and control group(51 patients). While the patients in the control group being treated solely with rh IFN-alpha 2 b,those in the observation group were given ALA-PDT in addition. The HPV-DNA load was detected with q RT-PCR before the treatment and 1 month and 3 months after the treatment respectively,and the negative conversion rate was analyzed 3 months after the treatment. The serum levels of TGF-beta 1 and IL-10 of the two groups were detected with enzyme-linked immunosorbent assay(ELISA)one month after the treatment.Results The HPV-DNA load in the observation group was lower than that in the control group 1 month and 3 months after the treatment. The negative conversion rates of the observation group and the control group were 80. 4% and 58. 8%(P<0. 05)respectively. The serum levels of TGF-beta 1 and IL-10 in the observation group were significantly higher than those in the control group(P<0. 05).Conclusions The combined therapy of Rh IFN-alpha 2 B and Ira-photodynamic will reduce the serum levels of TGF-beta 1 and IL-10 in patients with chronic cervicitis and persistent HPV infection and reduce the risk of cervical cancer.
作者
朱丽娟
周树林
江希萍
王丽昆
ZHU Li-juan;ZHOU Shu-lin;JIANG Xi-ping;WANG Li-kun(Department of Obstetrics and Gynecology,Changzhou First People's Hospital,Changzhou 213003,China;Department of Obstetrics and Gynecology,Maternal and Child Health Hospital of Jiangsu Province;Department of Dermatology,Affiliated Hospital of North China University of Science and Technology)
出处
《中国激光医学杂志》
CAS
2019年第6期324-328,共5页
Chinese Journal of Laser Medicine & Surgery
基金
华北理工大学科学研究基金项目（Z201129）
