摘要
目的 本研究旨在采集不同发育阶段幼龄SD大鼠的血液指标,建立相应的背景数据,为开展儿科药物安全性评价提供参考。方法 分别收集3、6、8、12和16周龄共计约200余只幼龄SD大鼠的血样,采用全自动血球计数仪、生化分析仪、测定相应指标。结果 从各指标参数结果看,6周龄组大鼠的血液指标与16周龄大鼠相比RBC、HGB、TP、BUN等大部分指标存在一定差别,12周龄组大鼠的血液指标与16周龄大鼠相比WBC、HGB、BUN等大部分指标无差异。结论 结果表明不同周龄阶段的幼龄SD大鼠的血液指标具有一定的时间相关性,建立不同时期的背景数据对于幼龄大鼠试验血液指标的统计分析和讨论具有一定的指导意义。
Objective The aim of this study is to collect physiological indicators of young SD rats at different developmental stages and establish relevant background data,to provide reference for the safety evaluation of pediatric drugs.Method The blood samples of about 200 young SD rats(3,6,8,12 and 16 weeks)were collected,and the corresponding indexes were determined by automatic blood cell counting instrument,biochemical analyzer.Result From the result of each index parameter,the blood indexes of 6-week-old rats were different from those of 16-week-old rats in RBC,HGB,TP,BUN,etc.The blood indexes of 12-week-old rats had no difference from those of 16-week-old rats in WBC,HGB and BUN.Conclusion The result show that the blood indexes of young SD rats at different stages have a certain time correlation.Establishing background data of different stages has a certain guiding significance for statistical analysis and discussion of blood indexes of young SD rats.
作者
孙超
黄维芝
孙俊梅
李晓菲
汤连升
SUN Chao;HUANG Weizhi;SNU Junmei;LI Xiaofei;TANG Liansheng(Shandong Institute of Pharmaceutical Industry,Shandong Provincial Key Laboratory of Chemical Drugs,Jinan 250101,China;Shandong College of Traditional Chinese Medicine Affiliated Hospital,Yantai 264199,China)
出处
《实验动物科学》
2019年第5期42-46,共5页
Laboratory Animal Science
