摘要
目的 研究环氧化酶抑制剂芬必得对腰椎间盘突出症的作用。方法 通过压迫背根神经节及移植自体尾椎髓核至腰椎对大鼠进行造模。COX(cyclooxygenase)抑制剂芬必得给药剂量为0. 051 g/kg体质量,连续28 d。对动物进行运动功能评分、机械刺激回缩反应测定、动物大腿周径测量及步态测量。给药28 d后取腰椎(L4~L6)及其神经用4%甲醛溶液固定后进行病理组织学检查,并取血清及神经根病变组织进行炎性因子浓度测定。结果COX抑制剂芬必得能够改善模型动物的动物运动功能评分、50%机械性缩足反射阈值、大腿周径比(造模侧/对侧)、坐骨神经功能指数(SFI),背根神经节及椎间盘组织病理改变,降低血清和病变位置的炎症因子(PGE2、COX-2、NO、IL-1β、IL-6)的水平。结论 COX抑制剂可改善大鼠腰椎间盘突出症模型症状。
Objective To study the effect of cyclooxygenase inhibitor Fenbid on lumbar disc herniation.Method Rats were modeled by oppressing the dorsal root ganglion and transplanting the autologous tail nucleus pulposus to the lumbar spine.The COX inhibitor Fenbid was administered at a dose of 0.051 g/kg for 28 days.Animals were scored for motor function,mechanical stimulation retraction tests,animal thigh circumference measurements,and gait measurements.After 28 days of dosing,lumbar vertebrae(L4-L6)and their nerves were fixed with 10% neutral formalin and histopathological examinations were performed.The serum and nerve root tissue under pressure were measured for inflammatory factor concentrations.Result The COX inhibitor,Fenbid,can improve the animals'motor function score,50% mechanical paw withdrawal threshold,thigh circumference ratio,sciatic nerve function index(SFI),the pathological changes of dorsal root ganglion and intervertebral d isc,reduce the levels of inflammatory factors(PGE2,COX-2,NO,IL-1β,IL-6)at the serum and lesion sites.Conclusion COX inhibitors improve the symptoms of lumbar disc herniation in rats.
作者
陈迪新
李瑞鹏
欧慧瑜
邵珍怡
郭秋平
CHEN Dixin;LI Ruipeng;OU Huiyu;SHAO Zhenyi;GUO Qiuping(Drug nonclinical evaluation and research center of Guangzhou pharmaceutical research institute,Guangzhou 510240,China;Postdoctoral Research Station of China Academy of Chinese Medical Sciences,Beijing 100700,China;Postdoctoral Research Station of Guangzhou Pharmaceutical Holdings Limited,Guangzhou 510130,China)
出处
《实验动物科学》
2019年第5期47-53,共7页
Laboratory Animal Science
基金
广州市博士后科研项目启动费
关键词
腰椎间盘突出症
退化
COX
PGE2
Lumbar disc herniation
degeneration
Cyclooxygenase
Prostaglandin E2
