摘要
Periconia igniaria Stdif10是从血散薯块根中首次分离得到的内生真菌,为了解该内生真菌的生物学活性,采用滤纸药膜法、菌丝生长速率法和带药培养基涂布法分别测定了该菌发酵产物不同溶剂萃取物的抗虫活性、抗真菌活性、抗细菌活性。结果表明,发酵产物的石油醚萃取物表现出较好的抗虫和抗菌活性。其中,对玉米象具有显著的触杀活性,处理72 h的LC 50值为2.2951 mg/cm 2。石油醚萃取物对玉米象还具有较好的驱避活性,处理48 h后在3.932、7.863、15.726 mg/cm 23个浓度梯度下对玉米象的驱避率均大于62%。石油醚萃取物对辣椒炭疽病菌、水稻胡麻叶病菌、茶轮斑病菌、甘蓝黑斑病菌、甘蔗凤梨病菌、烟草黑胫病菌、玉米大斑病菌、金橘砂皮病菌、贡柑链格孢菌9种供试植物病原真菌具有不同程度的抑制作用,其中对辣椒炭疽病菌、烟草黑胫病菌、金橘砂皮病菌3种病原真菌具有较好的抗菌活性,处理72 h的EC 50值分别为0.1173、0.0716、0.0547 mg/mL。石油醚萃取物对产气肠杆菌、铜绿假单孢菌、大肠杆菌、普通变形杆菌、伤寒沙门氏菌、金黄色葡萄球菌、蜡样芽孢杆菌、巨大芽孢杆菌、枯草芽孢杆菌、炭疽杆菌10种动物病原细菌具有显著的抗菌活性,处理24 h的MIC值为0.125~0.500 mg/mL。上述结果表明,血散薯内生真菌Periconia igniaria Stdif10次生代谢产物具有显著的抗虫和广谱的抗菌活性,且石油醚萃取物活性最佳(活性物质极性较低)。
Periconia igniaria Stdif10 is an endophytic fungus isolated from the root tubers of Stephania dielsiana Y.C.Wu for the first time.In order to understand the biological activity of P.igniaria Stdif10,the anti-insect,antifungal and antibacterial activities were determined with the extracts from petroleum ether layer,ethyl acetate layer and n-butanol layer of the fermentation products of this strain,by using the filter film method,mycelium growth rate method and toxic medium coating method.The results showed that petroleum ether extract possessed the best anti-insect and antimicrobial activity,having remarkable contact activity against Sitophilus zeamais with the LC 50 value at 2.2951 mg/cm 2 after treatment for 72 hours,and having a good repellent activity to S.zeamais.After treatment for 48 hours,the repellent rate of 3.932,7.863,15.726 mg/cm 2 concentration gradients of petroleum ether extract to S.zeamais was greater than 62%.Moreover,the petroleum ether extract showed inhibitory activity against Colletotrichum capsica,Cochliobolus miyabeanus,Pestalotiopsis theae,Alternaria oleracea,Ceratocystis paradoxa,Phytophthora parasitica var.nicotianae,Exserohilum turcicum,Diaporthe citri,Alternaria citri to some extent,and had best antifungal activity against Colletotrichum capsica,Phytophthora parasitica var.nicotianae and Diaporthe citri with the EC 50 values at 0.1173,0.0716 and 0.0547 mg/mL after treatment for 72 hours,respectively.The petroleum ether extract also exhibited significant inhibitory activity against Enterobacter aerogenes,Pseudomonas aeruginosa,Escherichia coli,Proteus vulgaris,Salmonella typhi,Staphyloccocus aureus,Bacillus cereus,Bacillus megaterium,Bacillus subtilis,Bacillus anthraci with the MIC values ranging from 0.125 to 0.500 mg/mL after treatment for 24 hours.The results in this study showed that endophytic fungus Stdif10 had remarkable anti-insect and broad-spectrum antimicrobial activities,and the petroleum ether extract had the most significant activity(the polarity of the active substance was low).
作者
林伟
骆海玉
邓业成
邓志勇
颜桢灵
张泽
王瑞昊
汤夏安
冯蓓蓓
LIN Wei;LUO Haiyu;DENG Yecheng;DENG Zhiyong;YAN Zhenling;ZHANG Ze;WANG Ruihao;TANG Xia’an;FENG Beibei(Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education/Guangxi Key Laboratory of Rare and Endangered Animal Ecology/College of Life Science,Guangxi Normal University,Guilin 541006,China)
出处
《河南农业科学》
北大核心
2020年第1期75-81,共7页
Journal of Henan Agricultural Sciences
基金
广西省自然科学基金项目(2018GXNSFAA281013,2016GXNSFAA380207,2016GXNSFBA380049)
2017年度广西高校中青年教师基础能力提升项目(2017KY0080)
广西珍稀濒危动物生态学重点实验室基金项目(GKN.17-A-02-02)
广西师范大学生态学博士点建设基金项目(EDPC2018002)
关键词
血散薯
内生真菌
触杀活性
驱避活性
抗真菌活性
抗细菌活性
Stephania dielsiana Y.C.Wu
Endophytic fungi
Contact toxicity
Repelling activity
Antifungal activity
Antibacterial activity
