摘要
本研究通过生化和分子遗传突变分析发现水稻(Oryza sativa)磷脂酶Dζ1(PLDζ1)参与盐胁迫反应过程。水稻PLDζ1在分蘖期和开花期的叶片中表达量较高,生化分析表明PLDζ1需要磷脂酰肌醇4,5-二磷酸激活,而在PLDα和PLDδ反应条件下的活性极低。PLDζ1缺失突变导致植株对盐胁迫的敏感性增加,在盐胁迫下pldζ1突变体株高和存活率均显著低于野生型(WT),并且pldζ1突变体植株钠离子(Na+)含量显著高于WT,其Na+吸收与转运相关基因HKT1表达量显著高于WT,而Na+流向液泡相关基因NHX1表达量则显著低于WT。这些结果表明水稻PLDζ1参与钠离子转运过程,从而正向调节植株的耐盐性。
This study showed that phospholipase Dζ1(PLDζ1)was involved in response to salt stress through biochemical analysis and genetic mutation in rice(Oryza sativa).The PLDζ1 transcript level were higher in leaves than those in other tissues at the tillering and heading stages.Biochemical analysis showed that rice PLDζ1 was activated by phosphatidylinositol 4,5-bisphosphate(PIP2)under PLDζreaction conditions but its activity was undetectable under PLDαand PLDβreaction conditions.Loss of PLDζ1 led to increase sensitivity to salt stress.The plant height and survival rate in the pldζ1 mutants were significantly reduced,as compared with wild type(WT)plants.Moreover,Na+content of pldζ1 mutant plants was much higher than that of WT.Loss of PLDζ1 led to an increased expression level of HKT1 involved in sodium influx and transport,as compared to WT.By contrast,the expression level of NHX1 related to vacuolar Na+/H+antiport was lower in the pldζ1 mutant than that in WT.These results suggest PLDζ1 plays a positive role in salt tolerance through modulating sodiumion transport in rice plants.
作者
吕玮鑫
周一梦
雷宵
洪月云
LÜWei-Xin;ZHOU Yi-Meng;LEI Xiao;HONG Yue-Yun(National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2019年第10期1516-1522,共7页
Plant Physiology Journal
基金
国家自然科学基金(31271514)~~
关键词
水稻磷脂酶Dζ1
盐胁迫
磷脂酸
钠离子转运
rice phospholipase Dζ1
salt stress
phosphatidic acid
sodium transport
