摘要
通过70℃(A组)、50℃(B组)、35℃(C组)热风干燥和冷冻干燥(D组)共四种方法分别对新鲜天麻进行干燥处理,然后采用蒽酮-硫酸法、考马斯G-250亮蓝法及高效液相色谱法分别测定天麻多糖、蛋白质及天麻素与对羟基苯甲醇的含量,探讨不同干燥温度对天麻主要有效成分含量的影响.结果表明:A、B、C、D处理组得到的天麻蛋白质含量分别为(1.110±0.017)%、(1.360±0.01)%、(2.000±0.014)%和(2.190±0.017)%,含量差异显著;其天麻多糖含量分别为(6.278±0.092)%、(7.331±0.048)%、(7.476±0.066)%和(7.682±0.025)%;其中A、D两组含量差异显著.其天麻素含量分别为(0.868±0.013)%、(0.287±0.009)%、(0.273±0.011)%和(0.156±0.007)%;其对羟基苯甲醇含量分别为(1.192±0.016)%、(1.314±0.016)%、(1.331±0.010)%和(1.565±0.009)%;其中A、D两组含量均差异显著.因此,不同干燥温度对天麻中主要有效成分有不同程度影响.干燥温度越低天麻中多糖和蛋白质含量越高;低温干燥有利于提高多糖、蛋白质和对羟基苯甲醇的含量,但天麻素的含量逐渐降低.传统的70℃烘干处理样品的天麻素和对羟基苯甲醇含量最高符合药用加工用途要求;四组样品中天麻素和对羟基苯甲醇含量之和均达到《中华人民共和国药典》的标准(即天麻素和对羟基苯甲醇含量之和不小于0.25%).
To study the effect of different drying temperatures on the content of main effective components in Gastrodia elata,fresh samples were dried at 70℃(group A),50℃(group B),35℃(group C)by hot air drying and freeze-drying(group D).The content of polysaccharide,protein,gastrodin and p-hydroxybenzenol were tested by anthrone sulfuric acid method,Coomassie G-250 brilliant blue method and HPLC,respectively.The results showed that the content of gastrodia elata protein obtained at A,B,C and D group was(1.10±0.017)%,(1.360±0.01)%,(2.000±0.014)%and(2.190±0.017)%,respectively.The content of gastrodia elata polysaccharides was(6.278±0.092)%,(7.331±0.048)%,(7.476±0.066)%and(7.682±0.025)%,respectively.The content of A and D groups was significantly different.The gastrodin content was(0.868±0.013)%,(0.287±0.009)%,(0.273±0.011)%and(0.156±0.007)%,respectively;the p-hydroxybenzyl alcohol content was(1.192±0.016)%,(1.314±0.016)%,(1.331±0.010)%and(1.565±0.009)%,respectively.A and D groups were significantly different.Thus,different drying temperatures have different effects on the main ingredients in Gastrodia elata.The lower the drying temperature,the higher the content of polysaccharides and protein in Gastrodia elata.The low temperature drying is beneficial to increase the content of polysaccharide,protein and p-hydroxybenzyl alcohol,but the content of gastrodin is gradually reduced meanwhile.The traditional 70℃drying treatment samples have the highest content of gastrodin and p-hydroxybenzyl alcohol in accordance with the requirements of medicinal processing;the sum of gastrodin and p-hydroxybenzyl alcohol content in the four samples reached the standard of the Pharmacopoeia of the People’s Republic of China(That is the sum of the content of gastrodin and p-hydroxybenzyl alcohol is not less than 0.25%).
作者
方伟
胡慧
刘慧芹
田玉桥
蒋忠权
伍贤进
FANG Wei;HU Hui;LIU Hui-qin;TIAN Yu-qiao;JIANG Zhong-quan;WU Xian-jin(College of Biological and Food Engineering,Huaihua University,Huaihua,Hunan 418008;Hunan Bestcome Traditional Medicine Co.,Ltd,Huaihua,Hunan 418000;Key Laboratory of Research and Utilization of Ethno medicinal Plant Resources of Hunan Province,Huaihua,Hunan 418008)
出处
《怀化学院学报》
2019年第11期1-5,共5页
Journal of Huaihua University
基金
湖南省战略性新兴产业科技攻关类项目“优质湖南雪峰天麻生产加工及其产业化关键技术研究”(2016GK4052)
湖南省双一流学科建设资助项目(201906)
关键词
天麻
干燥温度
有效成分
Gastrodia elata BI.
drying temperature
effective component
