摘要
以辣根过氧化物酶(HRP)为蛋白质模板分子,邻苯二胺(o-PD)为聚合单体,首先将预先羧基化的多壁碳纳米管(MWCNTs)通过阶跃电位法电沉积在玻碳电极上作为增敏材料,然后在该电极上电聚合含HRP的邻苯二胺电沉积液形成一层聚合膜,去除模板化合物后,制得对HRP具有特异性识别能力的分子印迹聚合物(MIPs)膜;利用聚邻苯二胺(POPD)的自探针效应构建了分子印迹电化学传感器.该传感器的响应电流与HRP浓度在1.0×10^-10~1.0×10^-5 mg/mL范围内有良好的线性关系,相关系数为0.991,检出限为1.5×10-11 mg/mL(S/N=3);该传感器的响应电流与H2O2浓度在4.0×10^-7~1.4×10^-5 mol/L范围内有良好的线性响应,相关系数为0.992,检出限为2.6×10^-7 mol/L(S/N=3),将该传感器用于实际样品H2O2的检测,回收率在91.2%~97.1%之间.建立了基于MIPs膜的HRP和H2O2双分析物传感器的制备方法,该方法可应用于酶及其酶促底物双分析物传感器.
Firstly,the pre-carboxylated multi-walled carbon nanotubes(MWCNTs)were electrodeposited on the surface of the glassy carbon electrode as sensitivity-enhanced materials.Then,a layer of polymeric film was formed on the modified electrode through the electropolymerization in the o-phenylenediamine(o-PD)monomer solution containing horseradish peroxidase template molecule.After removing the templates,the molecularly imprinted polymer membrane with specific recognition ability towards horseradish peroxidase was formed.The prepared molecularly imprinted electrochemical sensor had a good linear relationship with horseradish peroxidase(HRP)in the concentration range of 1.0×10^-10—1.0×10^-5 mg/mL,the correlation coefficient was 0.991,and the detection limit was 1.5×10^-11 mg/mL(S/N=3);and also exhibited a good linear response for H2O2 in the concentration range of 4.0×10^-7—1.4×10^-5 mol/L,with a correlation coefficient of 0.992 and a detection limit of 2.6×10^-7 mol/L(S/N=3).And this molecularly imprinted sensor was used for the detection of the actual sample,the recovery rate is between 91.2%and 97.1%.HRP and H2O2 bi-analyte sensor based on MIP film was successfully developed in this work.The developed method can also be applicable for enzyme and its enzymatic substrate bi-analyte sensor.
作者
滕渝
杨绍明
柏朝朋
张剑
TENG Yu;YANG Shaoming;BAI Chaopeng;ZHANG Jian(School of Material Science and Engineering,East China Jiaotong University,Nanchang 330013,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2020年第1期78-86,共9页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:21465012)资助~~
关键词
分子印迹聚合物
自探针
分子印迹电化学酶传感器
辣根过氧化物酶
过氧化氢
Molecularly imprinted polymer
Self-probe
Molecularly imprinted electrochemical enzyme sensor
Horseradish peroxidase
Hydrogen peroxide