摘要
本研究以甘蔗的3个杂交品种(ROC22、Co281、KQ01-1390)和3个原种(28NG16、57NG155、96NG16)为供试材料,研究低温胁迫对成熟期蔗株的形态、叶片的质膜相对透性、蔗茎的渗透调节物质含量及抗氧化酶活性等指标的影响,并对6个品种甘蔗耐寒性进行评价。结果表明:经−3℃夜间低温处理3 d后,甘蔗植株均出现叶片干枯、卷曲,生长点损伤或坏死变黑,蔗茎侧芽发黑、变软等症状,纵剖蔗茎可明显看到茎内组织呈水煮状,蔗叶相对电导率上升,蔗茎丙二醛(MDA)、脯氨酸(Pro)含量和过氧化氢酶(CAT)活性均上升,蔗茎超氧化物歧化酶(SOD)和过氧化物酶(POD)活性下降。通过隶属函数法综合评价,6个甘蔗品种耐寒能力依次为:KQ01-1390>Co281>ROC22>28NG16>57NG155>96NG16,可见供试材料中的甘蔗原种对低温胁迫反应敏感,耐寒性比杂交品种弱。
Three sugarcane hybrid varieties(ROC22,Co281,KQ01-1390)and three original breeding varieties(28NG16,57NG155,96NG16)were used to assess the ability of cold tolerance through investigating the effects of low temperature on mature sugarcane appearance,relative permeability of leave plasma,contents of osmotic adjustment substances and activities of antioxidant enzymes in sugarcane stems.Treated at−3℃for three days at nigh,symptoms such as withered and curly leaves,broken and black growth point,black and soft lateral bud appeared.Moreover,water boiling symptom in inner stem tissues was observed after ripping sugarcane stem.The relative electrical conductivity of the leaves increased,the contents of malonaldehyde(MAD)and proline(Pro),and the activities of catalase(CAT)increased,but the activity of superoxide dismutase(SOD)and peroxidase(POD)decreased in sugarcane stems under cold stress.Membership function method was used to assess the ability of cold tolerance,the results showed the order of cold tolerance was KQ01-1390>Co281>ROC22>28NG16>57NG155>96NG16,indicating that the original breeding were more sensitive to low temperature and showed weaker ability of cold tolerance than the hybrid varieties.
作者
全怡吉
樊仙
李如丹
杨绍林
邓军
QUAN Yiji;FAN Xian;LI Rudan;YANG Shaolin;DENG Jun(Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Kaiyuan,Yunnan 661600,China)
出处
《热带作物学报》
CSCD
北大核心
2020年第1期63-68,共6页
Chinese Journal of Tropical Crops
基金
国家糖料产业技术体系(No.CARS-170205)
关键词
甘蔗
品种
低温胁迫
生理特性
sugarcane
variety
low temperature stress
physiological character
