摘要
为探究土壤细菌群落对冻融期环境变化的响应机制,以新疆巴音布鲁克高寒湿地沼泽土壤为研究对象,基于高通量测序技术,研究冻融期高寒湿地土壤细菌群落结构和功能作用的变化。结果表明:细菌群落受控于冻融环境,物种多样性在冻结期显著降低(P<0.05);同时受土壤环境(土壤温度、土壤湿度、积雪厚度)和养分(土壤总碳、土壤总氮、碳氮比)的影响显著,解释贡献率分别为84.74%和73.91%,细菌群落结构发生生态适应性变化;初冻期、冻结期和融冻期,土壤细菌群落功能作用差异显著(P<0.05),主要体现在"代谢""信号转导"和"生存"三类功能基因含量的差异。冻融期高寒湿地土壤细菌群落结构和功能作用发生变化以增强群落的抗逆性,进化出较为完备的抗寒机制以适应冻融格局变化。
To explore the response mechanism of a soil bacterial community to environmental changes during a freeze-thaw period, variations in the soil bacterial community structure and function during this period in the Bayinbuluk alpine wetland were studied using highthroughput sequencing technology. Results showed that:The species diversity of the bacterial community was significantly reduced during the freeze period;The ecological adaptability of the bacterial community structure changed because of the influences of changes in the soil temperature, soil humidity, snow depth(explained 84.74% of the changes of bacterial community structure), soil total carbon, soil total nitrogen, and carbon-nitrogen ratio(explained 73.91% of the changes of bacterial community structure);The functional roles of soil bacterial communities were significantly different during the three periods. These differences mainly focused on the differences in three functional genes, namely "metabolism" "signal transduction" and "survival". The structure and function of the soil bacterial community in the alpine wetland changed during the freeze-thaw period, which enhanced the stress resistance of the community and thus, evolved a relatively complete cold-resistant mechanism to adapt to the freeze-thaw pattern changes.
作者
陈末
朱新萍
蒋靖佰伦
买迪努尔·阿不来孜
孙涛
贾宏涛
CHEN Mo;ZHU Xin-ping;JIANG Jing-bai-lun;Maidinuer·ABULAIZI;SUN Tao;JIA Hong-tao(College of Grassland and Environment Sciences,Xinjiang Agricultural University,Urumqi 830052,China;Key Laboratory of Soil and Plant Ecological Process in Xinjiang,Urumqi 830052,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2020年第1期134-142,共9页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(31560171,31960258)~~
关键词
冻融期
细菌群落
高通量测序
功能预测
freeze-thaw period
bacterial community
high-throughput sequencing
function prediction
