摘要
为明晰采收后向日葵盘精油的成分及其体外抗氧化活性,应用水蒸气蒸馏法提取采收后向日葵盘精油,利用气相色谱-质谱联用法(GC-MS)分析其化学成分,通过DPPH自由基和ABTS自由基清除法对精油的体外抗氧化活性进行测定。结果显示:从向日葵盘精油中共鉴定出49种化学成分(占精油总量91.16%),其中α-蒎烯(28.22%)、柠檬烯(6.55%)、α-蛇麻烯(4.99%)、反式-β-金合欢烯(3.58%)和莰烯(3.39%)为其主要成分。向日葵盘精油具有一定的体外抗氧化活性,其清除DPPH自由基、ABTS自由基的半数有效浓度(EC 50)值分别为0.47、0.30 g/L,明显高于阳性对照BHT的EC 50值(分别为8.21和6.16 mg/L)。
The essential oil of Helianthus annuus head which harvested in autumn was extracted by water-steam distillation,and it’s components was characterized by gas chromatography-mass spectrometry(GC-MS).The in vitro antioxidant activities of essential oil were studied by 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl(DPPH)and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical scavenging assay.The GC-MS analysis showed 49 kinds of chemical components(comprising 91.16%of the essential oil)were identified from the essential oil of H.annuus head,andα-pinene(28.22%),limonene(6.55%),α-humulene(4.99%),trans-β-farnesene(3.58%)and camphene(3.39%)were the main ingredients.The essential oil had some in vitro antioxidant activity,and the half effective concentration(EC 50)of the essential oil against DPPH and ABTS radical were 0.47 and 0.30 g/L,respectively,which significantly higher than that of BHT(8.21 and 6.16 mg/L).
作者
何昶毅
高潇涵
李泽文
刘玲玲
孙彤彤
陈小强
HE Changyi;GAO Xiaohan;LI Zewen;LIU Lingling;SUN Tongtong;CHEN Xiaoqiang(College of Life Science,Northeast Forestry University,Harbin 150040,China;College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040,China;College of Resources and Environment,Tibet Agriculture&Animal Husbandry University,Nyingchi 860000,China)
出处
《生物质化学工程》
CAS
北大核心
2020年第1期43-48,共6页
Biomass Chemical Engineering
基金
东北林业大学大学生创新训练项目(201910225336)
黑龙江省博士后科研启动金项目(LBH-Q15009)
黑龙江省自然科学基金项目(LH2019C006)
关键词
向日葵
精油
化学成分
抗氧化活性
Helianthus annuus L.
essential oil
chemical composition
antioxidant activity
