摘要
猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)又称为"猪蓝耳病",是一种由猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)引起的猪传染性疾病。本研究将采集到的2份疑似患有PRRS的流产胎儿的肺组织研磨后离心,取上清液接种猪肺泡巨噬细胞(porcine alveolar macrophage,PAM)盲传3代。通过RT-PCR方法和间接免疫荧光方法,对组织样品及细胞培养物进行了鉴定和分型。结果显示本研究成功从2份样品中分离到了类NADC30,且该毒株可以在PAM细胞上增殖。该研究结果为研究类NADC30毒株的重组机制、致病情况及研制预防疫苗提供参考。
In the present study,2 viruses were isolated from two aborted fetuses suspicious of porcine reproductive and respiratory syndrome(PRRS)by inoculating their lung homogenates onto porcine alveolar macrophages(PAMs).The isolated viruses were passed for 3 times on PAMs.The tissue samples and cell grown viruses were tested using RT-PCR and indirect immunofluorescent assay(IFA)and the presence of PRRS viruses was confirmed.Furthermore,the RT-PCR and IFA results indicated that these 2 viruses were similar to NADC30-like strains.
作者
邓跃
谭菲菲
王娟
常亚飞
陈喜波
王同燕
李向东
田克恭
DENG Yue;TAN Fei-fei;WANG Juan;CHANG Ya-fei;CHEN Xi-bo;WANG Tong-yan;LI Xiang-dong;TIAN Ke-gong(Henan Agricultural University,Zhengzhou 410005,China;National Research Center for Wterinary Medicine,Luoya ng 410003,China;Pulike Biological Engineering,Inc.,Luoyang 410000,China)
出处
《中国动物传染病学报》
CAS
北大核心
2020年第1期50-55,共6页
Chinese Journal of Animal Infectious Diseases
基金
国家自然科学基金(31490601)
关键词
猪繁殖与呼吸综合征
类NADC30
猪肺泡巨噬细胞
Porcine reproductive and respiratory syndrome virus
NADC30-like virus
porcine alveolar macrophage
