摘要
目的:探讨死亡受体5(DR5)、凋亡促进蛋白(FADD)在子宫肌瘤组织中的表达情况及临床意义。方法:选取2017年3月-2018年5月在本院进行子宫肌瘤切除术的72例患者作为研究对象,将手术切除的子宫肌瘤组织为肌瘤组织组,距离肿瘤边缘5 cm以外的瘤旁组织为瘤旁组织组。采用实时荧光定量聚合酶链反应(qRT-PCR)检测DR5和FADD的表达量;免疫组织化学法检测DR5和FADD的表达情况;用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法(TUNEL)统计细胞凋亡的情况,并分析DR5和FADD与细胞凋亡及两者的相关性。结果:肌瘤组织组DR5 m RNA、蛋白阳性表达率均高于瘤旁组织组(P<0.05),FADD mRNA、蛋白阳性表达率均低于瘤旁组织组(P<0.05);肌瘤组织组凋亡指数(AI)低于瘤旁组织组,增殖指数(PI)高于瘤旁组织组(P<0.05);在子宫肌瘤组织中,DR5的表达与AI呈负相关(P<0.05),与PI呈正相关(P<0.05),FADD的表达与AI呈正相关(P<0.05),与PI呈负相关(P<0.05);DR5与FADD呈负相关(P<0.05)。结论:在子宫肌瘤组织中,DR5高表达,FADD低表达,且两者均与AI、PI相关性明显,可能共同参与子宫肌瘤的发生发展。
Objective: To investigate the expressions of death receptor 5(DR5) and apoptosis promoting protein(FADD) in uterine leiomyoma and their clinical significances. Methods: 72 patients who underwent hysteromyomectomy in our hospital from March 2017 to May 2018 were selected as the study subjects. The uterine leiomyoma tissue resected surgically was used as the myoma tissue group and the adjacent tissue 5 cm away from the edge of the tumor was used as the adjacent tissue group. Real-time fluorescence quantitative PCR(qRT-PCR) was used to detect the expressions of DR5 and FADD, the expressions of DR5 and FADD were detected by immunohistochemistry, TUNEL method was used to count apoptosis, and the correlations between DR5, FADD and apoptosis and the correlation between them was analyzed. Results: The positive expression rates of DR5 m RNA and protein in the myoma tissue group were higher than those in the adjacent tissue group(P<0.05), and the positive expression rates of FADD m RNA and protein were lower than those in the adjacent tissue group(P<0.05). AI in the myoma tissue group was lower than that in the adjacent tissue group, PI was higher than that in the adjacent tissue group(P<0.05). In uterine leiomyoma, the expression of DR5 was negatively correlated with AI(P<0.05), and positively correlated with PI(P<0.05), the expression of FADD was positively correlated with AI(P<0.05), and negatively correlated with PI(P<0.05). DR5 was negatively correlated with FADD(P<0.05).Conclusions: In uterine leiomyoma, DR5 is highly expressed, FADD expression is low, and they are negatively correlated. Both of them are significantly correlated with AI and PI, which may participate in the occurrence and development of uterine leiomyoma.
作者
唐亮
王敏
张利军
王梅
肖燕
TANG Liang;WANG Min;ZHANG Lijun;WANG Mei;XIAO Yan(Department of Gynecology,Panzhihua City Maternal and Child Care Hospital,Panzhihua 617000,Sichuan Province,China;Department of Laboratory,Panzhihua City Maternal and Child Care Hospital,Panzhihua 617000,Sichuan Province,China)
出处
《国际妇产科学杂志》
CAS
2020年第1期37-40,I0001,共5页
Journal of International Obstetrics and Gynecology
基金
攀枝花市科学资助项目（13SHFZ0012）
