摘要
目的采用高效液相色谱法测定保健食品五味子猴菇口服液中葛根素的含量,为进一步提高其质量控制提供依据。方法采用Agilent ZORBAX Eclipse Plus C18(4.6mm×250mm,5µm)色谱柱,以乙腈-0.05%磷酸溶液为流动相,检测波长为250nm。结果葛根素在8.48106~42.4053μg/mL范围内与峰面积呈良好线性关系(r>0.9990);平均加样回收率(n=6)分别为101.47%。结论建立了五味子猴菇口服液中葛根素的含量测定的方法,方法重复性良好,为该保健食品的产品质量控制提供依据。
Objective To determine the contents of puerarin in health food schisandrae hericium erinaceus oral liquid by high performance liquid chromatography to provide the reference for further improving the quality control.Methods An Agilent ZORBAX Eclipse Plus C18(4.6mm×250mm,5µm)column was used with acetonitrile-0.05%phosphoric acid as the mobile phase.The detection wavelength was 250nm.Results Puerarin showed a good linear relationship with the peak area in the range of 8.48106-42.4053μg/mL(r>0.9990).The average sample recovery rate(n=6)was 101.47%.Conclusion The method for determination of puerarin in schisandrae hericium erinaceus oral liquid is established.The method has good repeatability and provides the basis for the quality control of the health food.
作者
赵昱玮
盛雪
唐超
刘健
刘秀华
曹扬
高其品
ZHAO Yuwei;SHENG Xue;TANG Chao;LIU Jian;LIU Xiuhua;CAO Yang;GAO Qipin(Jilin Jice Test Technology Co.,Ltd.,Jilin,Changchun 130117,China;Changchun University of Chinese Medicine,Jilin,Changchun 130117,China)
出处
《中国医药科学》
2020年第1期39-42,共4页
China Medicine And Pharmacy
基金
吉林省中医药科技项目（2019155）
吉林省科技发展计划项目（20180623037TC）
关键词
高效液相色谱法
五味子猴菇口服液
葛根素
含量测定
High performance liquid chromatography
Schisandrae hericium erinaceus oral liquid
Puerarin
Determination of contents
