摘要
磷是植物生长所必需的重要元素之一,土壤中的磷大都以难溶性磷的形式存在,难以被植物吸收利用。为提高盐碱地中磷的利用率,从天津滨海新区盐碱地土壤中筛选解磷细菌,在无机磷固体平板上分离得到一株能产生明显溶磷圈的菌株LQH-7,其溶磷指数为2.86,形态学、生理生化和基于16S rDNA基因序列的分子鉴定结果表明,LQH-7为维罗尼假单胞菌,与Pseudomonas veronii CIP 104663的同源性为99.65%。在初始pH为8.0,NaCl质量分数为0.5%的无机磷培养基中,30℃摇瓶发酵60 h,菌株LQH-7发酵液的pH为4.30,解磷量最高可达850.1 mg/L,说明该菌株在盐碱地土壤生物修复方面具有潜在的应用价值。
Phosphorus is one of the important elements for plant growth.Plants cannot absorb enough phosphorus when a large amount of phosphorus in the soil exists in the form of insoluble state.To increase the utilization efficiency of phosphorus in saline-alkali,phosphate solubilizing bacteria were isolated from saline-alkali soil in Binhai New Area of Tianjin.Strain LQH-7,producing distinct soluble phosphorus circle with SPI 2.86,was isolated in inorganic phosphorus solid plate.Morphology,physiological-biochemical index and phylogenetic analysis based on 16 S rDNA gene sequences showed that strain LQH-7 belonged to Pseudomonas veronii,exhibiting the highest sequence similarity with P.veronii CIP 104663(99.65%).In inorganic phosphorus medium,under the fermentation conditions in which the initial pH was 8.0,NaCl content was 0.5%,temperature was 30℃and fermentation time was 60 h,pH of strain LQH-7 fermentation broth reached 4.30 and the phosphate-solubilizing quantity reached its highest value,850.1 mg/L,suggesting that LQH-7 is a potent candidate for bioremediation of saline-alkali soil.
作者
刘希旻
杨海霞
黄灵曦
李晓雁
权桂芝
杨红澎
黄海东
LIU Xi-min;YANG Hai-xia;HUANG Ling-xi;LI Xiao-yan;QUAN Gui-zhi;YANG Hong-peng;HUANG Hai-dong(College of Agronomy and Resources Environmentt,Tianjin Agricultural University,Tianjin 300384,China;College of Food Science and Bioengineering,Tianjin Agricultural University,Tianjin 300384,China)
出处
《天津农学院学报》
CAS
2019年第4期49-53,共5页
Journal of Tianjin Agricultural University
基金
国家级大学生创新创业训练计划项目(201910061002)
关键词
解磷菌
维罗尼假单胞菌
菌种鉴定
解磷能力
phosphate solubilizing bacteria
Pseudomonas veronii
identification of bacteria
phosphate solubilizing ability
