摘要
二点委夜蛾Athetis lepigone是夏玉米苗期的一种重要害虫。为了对该虫进行有效预测预报,对其卵巢发育的形态变化过程进行了观察并分级。将室内饲养的二点委夜蛾雌蛹按不同日龄进行解剖,雌成虫按每12 h进行解剖,观察不同时间卵巢的发育情况,并测量卵巢管长度及第一卵室长度和宽度。二点委夜蛾的卵巢形成于蛹期并在蛹期完成其基本形态发育过程,具有1对卵巢,各由4根卵巢管组成。根据有无卵粒和卵黄沉积情况可将其卵巢发育分为5个时期:未分化期(Ⅰ级);乳白透明期(Ⅱ级);卵黄沉积期(Ⅲ级);成熟待产期(Ⅳ级);产卵末期(Ⅴ级)。从发育进度来看,蛹期是卵巢大小和卵巢管长度快速增长的阶段。蛹期第3天开始出现卵巢形态分化,卵粒发生始见于蛹期第5天至第6天,到蛹期第7天卵巢形态结构即发育完成。羽化后雌虫卵巢继续发育,卵粒不断膨大、卵巢管逐渐增长并出现卵黄沉积,至羽化后48~60 h卵巢管第一卵室长和宽及卵巢管的长度均已达到最大值,分别为0.58 mm±0.01 mm、0.55 mm±0.01 mm以及16.91 mm±0.58 mm。本研究明确了二点委夜蛾卵巢发育的形态变化过程和分级标准,为二点委夜蛾的生殖生理学研究提供理论基础,并为该虫的预测预报提供依据。
Athetis lepigone(M schler)is an important pest of summer corn at seedling stage.In order to effectively predict the occurrence of A.lepigone,the morphological changes and the ovarian grades were investigated in this study.Female pupae and adults reared in the laboratory were dissected according to the daily age and every 12 hours,respectively.The developmental characteristics of ovaries in different stages were observed.The length of the ovarian tube,the length and width of the first egg chamber were measured.The results indicated that the ovary of A.lepigone,consisting of paired ovaries with each ovary consisting of four ovarian tubes,was formed in the pupa stage and completed its basic morphological development during this period.The ovarian development of A.lepigone was divided into five stages according to external ovarian morphological characteristics,appearance or disappearance of an egg chamber and yolk deposition,including the initial period(Stage I),transparent period(StageⅡ),yolk deposition period(StageⅢ),egg maturation period(StageⅣ),and late egg-laying period(StageⅤ).During the overall developmental progress,the pupal stage underwent a rapid growth in the ovarian size and ovarian tube length.Ovarian differentiation began on the 3rd day of the pupal stage.Eggs began to appear on the 5th to 6th day of the pupal stage,and the morphological structure of the ovary was basically completed on the 7th day of the pupal stage.After eclosion,with continued development of the ovary,the egg continued to expand,and the ovarian tube gradually grew and yolk deposition began to appear.The length and width of the first egg chamber and the length of the ovarian tube reached the maximum at 48-60 hours after emergence,which were 0.58 mm±0.01 mm,0.55 mm±0.01 mm and 16.91 mm±0.58 mm,respectively.In this study,we observed the morphological change during the ovarian development of A.lepigone and proposed a classification method,which provided a scientific reference for forecasting and integrated management of this pest.
作者
罗丹
周亚媛
何运转
LUO Dan;ZHOU Yayuan;HE Yunzhuan(College of Plant Protection,Hebei Agricultural University,Baoding 071000,China)
出处
《植物保护》
CAS
CSCD
北大核心
2020年第1期140-144,168,共6页
Plant Protection
基金
国家公益性行业(农业)科研专项(201303026)
河北省科技厅项目(112203010)
河北农业产业技术体系(HBCT2018060204)
关键词
二点委夜蛾
卵巢发育
卵巢解剖
分级
Athetis lepigone
ovarian development
ovarian dissection
classification
