摘要
半乳糖凝集素(Galectins)属于一种多功能凝集素家族,在机体抵抗微生物的感染中起重要作用。本研究利用cDNA末端快速扩增(rapid-amplification of cDNA ends,RACE)技术克隆获得马氏珠母贝galectin-4(PmGal-4)基因cDNA的全长,并对其序列进行分析。PmGal-4基因cDNA全长1071 bp,5′非翻译区(5′UTR)长75 bp,3′非翻译区(3′UTR)长72 bp;其开放阅读框(open reading frame,ORF)长度为924 bp,编码307个氨基酸组成的前体肽,理论分子量约为34.6 kD,理论等电点为8.80。多序列比对结果显示各物种间galectin-4具有高保守性。序列分析结果显示PmGal-4的氨基酸序列具有典型富含半胱氨酸(cysteine-rich domain,CRD)的结构域。实时荧光定量PCR分析表明,PmGal-4基因在马氏珠母贝所检测的组织中呈组成型表达特征,但在闭壳肌和外套膜中表达量最高。上述结果表明PmGal-4基因可能参与了马氏珠母贝多种生理功能,特别是在免疫防御反应中具有重要的作用。
Galectin is a family of multifunctional lectins that play an important role in the body’s resistance to microbial infections.The full length of galectin-4(PmGal-4)gene was obtained using rapid amplification of cDNA ends(RACE)technology,and its sequence was analyzed.The results showed that the cDNA of PmGal-4 was 1071 bp in length,including 75 bp in 5’UTR,and 72 bp in 3’UTR,and 924 bp in open reading frame(ORF),which encoding a precursor with 307 amino acids,The predicted molecular weight and isoelectric point is 34.6 kD and 8.80,respectively.Multiple sequence alignments showed high conservation of galectin-4 among various species.The domain prediction results showed that PmGal-4 has a typical CRD domain in its sequence.Real-time fluorescence quantitative PCR results showed that PmGal-4 gene was expressed in all the tested tissues of P.fucata martensii,and the expression was highest in both of the adductor muscle and mantle.The results of this study showed that PmGal-4 gene may play an important role in various biological process,especially in immune defense response of Pinctada fucata martensii.
作者
何军军
吴羽媛
梁海鹰
阮尧
杜晓东
He Junjun;Wu Yuyuan;Liang Haiying;Ruan Yao;Du Xiaodong(College of Fisheries,Guangdong Ocean University,Zhanjiang,524088)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2019年第11期4919-4927,共9页
Genomics and Applied Biology
基金
国家自然科学基金(31472306)
广东省海港建设与渔业产业发展专项(A201608B15)
广东省科技计划项目(2012A031100010)共同资助
