摘要
目的建立HPLC-ELSD法测定氨糖软骨素中硫酸软骨素和D-盐酸氨基葡萄糖的含量。方法采用HPLC-ELSD法,色谱柱为Waters Xselect HSS T3(5μm,4.6 mm×250 mm);流动相为乙腈-水-三乙胺(10∶90∶0.1);流速为0.5 mL·min^-1,柱温为30℃;漂移管温度为90℃;载气流速为3.0 L·min^-1。结果硫酸软骨素及D-盐酸氨基葡萄糖进样量分别在0.22~2.19μg及0.40~3.96μg与峰面积线性关系良好,硫酸软骨素的平均回收率为96.6%,RSD为2.8%;D-氨基葡萄糖回收率的平均回收率为99.4%,RSD为2.3%。结论本方法准确,可靠,可用于氨糖软骨素中硫酸软骨素及D-盐酸氨基葡萄糖的含量测定。
Objective To determine chondroitin sulfate and D-glucosamine hydrochloride in ammonia sugar chondroitin capsules by HPLC-ELSD.Methods HPLC-ELSD was used.A Waters Xselect HSS T3(5μm,4.6 mm×250 mm)column was used.The flow rate of the mobile phase consisting of acetonitrilewater-triethylamine(10∶90∶0.1)was 0.5 mL·min^-1.The column temperature was 30℃.The drift tube temperature was 90℃.The flow rate of the carrier gas was 3.0 L·min^-1.Results The linearity was good when chondroitin sulfate ranged from 0.22 to 2.19μg and D-glucosamine hydrochloride ranged from 0.40 to 3.96μg.The average recovery of chondroitin sulfate was 96.6%(RSD=2.8%).The average recovery of D-glucosamine was 99.4%(RSD=2.3%).Conslusion This method can determine the content of chondroitin sulfate and D-glucosamine in glucosamine chondroitin accurately.
作者
蔡泽宇
石骏
杨政芳
郭姣姣
王正雄
CAI Ze-yu;SHI Jun;YANG Zheng-fang;GUO Jiao-jiao;WANG Zheng-xiong(Hubei Hongyuan Pharmaceutical Technology Co.Ltd.,Wuhan R&D Center,Wuhan 430070)
出处
《中南药学》
CAS
2019年第12期2142-2145,共4页
Central South Pharmacy
