摘要
南瓜属(Cucurbita)作物包括中国南瓜(C.moschata D.)、印度南瓜(C.maxima D.)和美洲南瓜(C.pepo L.)等许多重要蔬菜,在世界范围内广泛栽培,具有重要的经济价值。目前,南瓜属作物遗传转化技术普遍存在转化效率低、重复性差、假阳性和嵌合体干扰等诸多问题。随着分子生物学的迅猛发展,南瓜属作物遗传改良和基因功能验证亟需建立高效稳定的遗传转化体系。前人在南瓜离体再生、花粉管通道和农杆菌介导等转化方法上做过不少探索,普遍认为以农杆菌介导的子叶节离体再生是最具潜力的转化体系。笔者对现有的研究基础进行综述,为促进其在南瓜属作物遗传改良上的应用提供参考。
Cucurbita crop includes many important vegetables,such as C.moschata D.,C.maxima D.and C.pepo L.,which are widely cultivated worldwide and have important economic values.At present,there are many problems of genetic transformation in Cucurbita,such as low efficiency,poor repeatbility,and interference of false-positive and chimeric.As the rapid development of molecular biology,it is urgent to establish an efficient and stable genetic transformation system for genetic improvement and gene function verification.Many researches have been conducted on the methods of pumpkin regeneration in vitro,pollen tube pathway and Agrobacterium-mediated transformation.It is generally believed that Agrobacterium-mediated cotyledon node regeneration is the most potential transformation system.Here we summarize the research progress in this field,providing references to promote its application in the genetic improvement of Cucurbita crops.
作者
陈学进
韩涛
姜立娜
郭卫丽
周俊国
CHEN Xuejin;HAN Tao;JIANG Lina;GUO Weili;ZHOU Junguo(Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement/School of Horticulture and L andscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China)
出处
《中国瓜菜》
CAS
北大核心
2020年第1期1-6,共6页
China Cucurbits And Vegetables
基金
河南省自然科学基金项目（162300410110）
河南科技学院高层次人才科研启动项目（2014023）
关键词
南瓜属
花粉管通道法
农杆菌
转基因
离体再生
Cucurbita
Pollen tube mediated gene transfer
Agrobacterium
Transgenesis
Regeneration in vitro
