摘要
研究乳酸菌发酵大麦提取物(LFBE)对饮食诱导的肥胖大鼠胰岛素抵抗的干预作用及其机制。通过饲喂高脂饲料的方法建立营养型肥胖模型大鼠,在饲喂模型大鼠高脂饲料的同时,分别灌胃LFBE(1 000 mg/kg bw)和水(4 mL/kg bw) 8周后,测定其体重、血脂、炎症因子等指标。结果显示,与模型对照组相比,LFBE能显著降低营养型肥胖大鼠的体重、血脂和胰岛素水平,提高糖耐量,说明LFBE可以减缓肥胖大鼠体重的增加,改善糖代谢紊乱和胰岛素抵抗症状。同时,LFBE可抑制促炎因子的表达,抑制NF-B和c-Jun氨基末端激酶(JNK)/p38介导的丝裂原活化蛋白激酶(MAPK)信号通路,从而改善胰岛素抵抗症状。研究结果表明,LFBE具有干预胰岛素抵抗的作用,其作用机制主要与抑制MAPK(JNK、p38)的磷酸化以及NF-B活性有关。
To study the effect of fermented barley extract with Lactobacillus plantarum dy-1(LFBE) on insulin resistance in high-fat diet(HFD)-induced obese rats. We induced the obese rat model by feeding high fat diet, then treated these rats with LFBE(1 000 mg/kg bw) and water(4 ml/kg bw) respectively for 8 weeks, and determinated the levels of body weight, serum lipid profiles and insulin levels, glucose tolerance and others. LFBE could reduce the body weight, the levels of serum lipid profiles and insulin, improve glucose tolerance. So, LFBE could lower rate of increase of obese rats’ body weights, reverse the disorders of glucose metabolism and ameliorate the symptoms of insulin resistance in obese rats. At the same time, LFBE can inhibit expression of pro-inflammatory factors and the NF-κB and the JNK/p38 mediated MAPK signaling pathway in obese rats, and then ameliorate the symptoms of insulin resistance. The mechanism was related with LFBE can inhibit the phosphorylation of MAPK and NF-κB.
作者
张家艳
肖香
周兴华
董英
Zhang Jiayan;Xiao Xiang;Zhou Xinghua;Ying Dong(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,Jiangsu)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2020年第2期9-17,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
江苏高校优势学科建设工程项目
