摘要
目的探讨八聚体结合蛋白4(Oct4)、Nanog、β-连环蛋白(β-catenin)在喉癌组织中的表达情况及临床意义。方法收集71例喉癌患者的喉癌组织和对应的癌旁组织,采用免疫组织化学染色法检测两种组织中Oct4、Nanog、β-catenin的表达情况,分析喉癌组织中Oct4、Nanog、β-catenin的表达情况及与喉癌患者临床特征的关系,并分析喉癌患者预后的影响因素。结果癌旁组织中Oct4、Nanog、β-catenin的阳性表达率均明显低于喉癌组织,差异均有统计学意义(P﹤0.01)。中低分化、T3~4期、有淋巴结转移的喉癌患者喉癌组织中Oct4、Nanog、β-catenin的阳性表达率均高于高分化、T1~2期、无淋巴结转移的患者(P﹤0.05)。β-catenin和Oct4表达的一致性较好(Kappa=0.633,P=0.000),β-catenin和Nanog表达的一致性较差(Kappa=0.381,P=0.001)。喉癌组织中Oct4、Nanog、β-catenin阳性表达患者的5年生存率均低于Oct4、Nanog、β-catenin阴性表达的患者(P﹤0.05)。TNM分期、淋巴结转移情况及β-catenin、Oct4、Nanog表达情况均是喉癌患者预后的独立影响因素(P﹤0.05)。结论Oct4、Nanog和β-catenin在喉癌组织中均异常表达,三者可能协同参与了喉癌的发生和发展过程,联合检测有助于预测和评估患者预后。
Objective To investigate the expression and clinical significance of octamer-binding protein 4(Oct4),Nanog andβ-catenin in laryngeal carcinoma.Method A total of 71 cases of laryngeal carcinoma were included in the analysis,the laryngeal carcinoma tissues and surrounding tissues were collected.Immunohistochemical staining was used to detect the expression of Oct4,Nanog andβ-catenin in these specimens,to determine the correlation between their expression and the clinical characteristics of laryngeal carcinoma,besides,the prognostic factors were identified.Result The positive expression rates of Oct4,Nanog andβ-catenin in laryngeal carcinoma tissues were significantly higher compared to normal tissues,with statistically significant difference observed(P<0.01).The positive expression rates of Oct4,Nanog andβ-catenin in laryngeal carcinoma with low or moderate differentiation,clinical stage T3-4,and positive lymph node metastasis were higher compared to those with high differentiation,stage T1-2,and negative lymph node metastasis(P<0.05).The consistency ofβ-catenin and Oct4 expression were relatively good(Kappa=0.633,P=0.000),and it was poor betweenβ-catenin and Nanog(Kappa=0.381,P=0.001).The 5-year survival rates of patients with positive expressions of Oct4,Nanog andβ-catenin in laryngeal carcinoma were significantly lower than that of patients with negative expression(P<0.05).TNM stage,lymph node metastasis,and expression ofβ-catenin,Oct4 and Nanog were independent prognostic factors for laryngeal carcinoma(P<0.05).Conclusion Abnormal expression of Oct4,Nanog andβ-catenin are present in laryngeal carcinoma,the three markers may be jointly involved in the occurrence and development of laryngeal carcinoma,suggesting that combined detection of them might be useful to predict and assess the prognosis of patients.
作者
李冬雷
张旭宇
肖跃华
刘伟
张美伦
LI Donglei;ZHANG Xuyu;XIAO Yuehua;LIU Wei;ZHANG Meilun(Department of Oncology,Baoding Second Central Hospital,Baoding 072750,Hebei,China;Department of Otolaryngology,Baoding Second Central Hospital,Baoding 072750,Hebei,China)
出处
《癌症进展》
2020年第2期148-151,175,共5页
Oncology Progress
