摘要
目的:探讨folliculin(FLCN)基因在子宫颈鳞癌组织中的表达及其对子宫颈鳞癌细胞增殖和凋亡的影响。方法:采用qRT-PCR和免疫组化法分别检测正常宫颈组织和子宫颈鳞癌组织中FLCN mRNA和蛋白的表达;在子宫颈鳞癌SiHa细胞中敲减或过表达FLCN后,qRT-PCR检测FLCN mRNA表达,采用CCK-8检测各组细胞增殖活力,采用TUNEL和磷酸化组蛋白H3(PH3)染色,观察各组细胞凋亡和增殖情况。结果:与对照组相比,宫颈鳞癌组中FLCN mRNA和蛋白表达水平均显著降低(P均<0.05)。FLCN siRNA沉默后细胞增殖活力增强、凋亡减弱,PH3蛋白阳性比例增加,TUNEL阳性细胞比例减少(P<0.05或0.01)。过表达FLCN细胞增殖活力减弱、凋亡增强,PH3蛋白阳性细胞比例减少,TUNEL阳性细胞比例增加(P均<0.01)。结论:FLCN基因在宫颈鳞癌组织中呈低表达,其可抑制SiHa细胞增殖并促进其凋亡。
Objective:To investigate the expression of folliculin(FLCN)in cervical squamous cancer tissue and its effect on the cell proliferation and apoptosis in cervical carcinoma.Methods:The mRNA and protein expressions of FLCN in cervical squamous cancer tissue were detected by qRT-PCR and immunohistochemical technique.After overexpression or knocking down of FLCN in cervical squamous cancer SiHa cells,the FLCN mRNA expression was detected by qRT-PCR,the cell proliferation was evaluated by CCK-8 and phosphorylated histone H3(PH3)staining,the cell apoptosis was measured by TUNEL.Results:Compared with the control group,FLCN mRNA and protein level expression in cervical squamous cancer groups were significantly reduced(P<0.05).After FLCN siRNA was transfected,cell proliferation increased and apoptosis decreased,PH3 protein positive ratio increased,TUNEL positive cell ratio decreased(P<0.05 or<0.01).Afer over-expression of FLCN,cell proliferation was inhibited and apoptosis was enhanced,the proportion of PH3 protein-positive cells decreased,and the proportion of TUNEL-positive cells increased(all P<0.01).Conclusion:FLCN gene was low-expression in cervical squamous cancer tissue,which could inhibit proliferation and promote apoptosis of SiHa cells.
作者
张煜
杨佩芳
陈霞
栾晓瑾
颜一丹
于骏
ZHANG Yu;YANG Pei-fang;CHEN Xia;LUAN Xiao-jin;YAN Yi-dan;YU Jun(Department of Obstetrics and Gynaecology, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)
出处
《江苏大学学报（医学版）》
CAS
2020年第1期63-67,共5页
Journal of Jiangsu University：Medicine Edition
