摘要
目的观察温肾活血方对对RCS大鼠视网膜变性损伤的保护作用。方法RCS大鼠按照4雌2雄进行交配得到纯合子胎鼠RCS(rdy-/-,p-/-)24只(雌雄各半),另8只SD大鼠作为正常对照组。纯合子RCS大鼠在岀生后14天随机分为3组,即模型对照组、低剂量中药组、高剂量中药组,每组8只。高剂量中药组、低剂量中药组予不同浓度的温肾活血方灌胃治疗,每日1〜2次,模型组及空白组予蒸懈水灌胃治疗,连续治疗28天。HE染色观察视网膜各层细胞形态和结构的变化,并计数视网膜外核层细胞核数目变化;TUNEL染色观察视网膜外核层细胞凋亡情况。结果HE染色显示,RCS大鼠各组视网膜外核层逐渐变薄,外核层细胞数减少。温肾活血方组在治疗后视网膜形态改善,外核层细胞核层数增加。外核层细胞数量各组间比较差异有显著统计学意义(P<0.05),且高剂量中药组优于低剂量中药组(P<0.05)。TUNEL检测结果显示外核层细胞凋亡计数各组差异有统计学意义(均为P<0.05),且高剂量中药组优于低剂量中药组(P<0.05)。结论温肾活血方对RCS大鼠视网膜变性损伤、感光细胞凋亡具有一定的保护作用,可能延缓病情的发展过程。
Objective To observe the protective effect of Wenshen Huoxue recipe on retinal degeneration injury in RCS rats.Methods RCS rats were mated with(4 males and 2 males)to obtain homozygous RCS(rdy-/-,p-/-).On the 14th day after birth,total 24 RCS rats(half male and half female)were randomly divided into three groups:model control group,low-dose traditional Chinese medicine group and high-dose traditional Chinese medicine group with 8 rats in each group,and the other 8 SD rats were used as normal control group.In the high-dose traditional Chinese medicine group,the low-dose traditional Chinese medicine group was given different concentrations of Wenshen Huoxue recipe intragastrically,once a day,and the model group and the normal control group were treated with distilled water for 28 days.The morphological and structural changes of the retinal cells in each layer were observed by HE staining,and the number of cells in the outer retinal nuclear layer was counted.The apoptosis of the outer retinal nuclear layer was observed by TUNEL staining.Results At the end of the course of treatment,compared with SD rats,the outer nuclear layer of RCS rats became thinner and the number of cells in outer nuclear layer decreased.In the Wenshen Huoxue recipe group,the retinal morphology was improved and the number of nuclear layers in the outer nuclear layer was increased after treatment.There was a significant difference in the number of cells in the external nuclear layer(P<0.05),and the high-dose traditional Chinese medicine group was better than the low-dose traditional Chinese medicine group(P<0.05).The results of the TUNEL test showed that there was a significant difference in the apoptotic count of the external nuclear layer(P<0.05),and the high-dose traditional Chinese medicine group was superior to that of the low-dose traditional Chinese medicine group(PVO.05).Conclusion Wenshen Huoxue recipe has certain protective effect on retinal degeneration and apoptosis in RCS rats,which may delay the development of the disease.
作者
向圣锦
李佳
窦仁慧
姜小涵
李柏村
Xiang Sheng jin;Li Jia;Dou Renhui;Jiang Xiaohan;Li Bocun(Eye Hospital,Wenzhou Medical University,Wenzhou,Zhejiang,325027;Hubei University of Traditional Chinese Medicine,Wuhan,Hubei,430061;Eye Hospital,Wenzhou Medical University,Hangzhou,Zhejiang,310020)
出处
《中医眼耳鼻喉杂志》
2019年第4期186-189,共4页
Journal of Chinese Ophthalmology and Otorhinolaryngology
基金
浙江省自然科学基金资助项目(编号:LY15H270015)
温州市科技计划项目(编号:2016Y0379)。
关键词
温肾活血方
视网膜色素变性
感光细胞
凋亡
Wenshen Huoxue recipe
Retinal pigmentosa
Photoreceptor cells
Apoptosis
