摘要
目的探讨Ki67、CD34、D2-40、PD-L1免疫组化标志物及BRAF V600E基因突变预测甲状腺乳头状癌(papillary thyroid carcinoma,PTC)颈部淋巴结转移的价值。方法应用免疫组织化学法和实时荧光定量PCR技术分别检测Ki67、CD34、D2-40、PD-L1和BRAF V600E基因在147例PTC组织中的表达。比较各分子标志物在伴颈部淋巴结转移和不伴颈部淋巴结转移PTC病灶中的表达差异,采用Logistic回归分析PTC伴颈部淋巴结转移的独立相关因子。结果Ki67、CD34、D2-40、PD-L1均与PTC颈部淋巴结转移具有相关性(P均<0.05),BRAF V600E基因突变与PTC颈部淋巴结转移无明显相关性(P>0.05)。Ki67和CD34是PTC颈部淋巴结转移的独立相关因素(P均<0.05),OR值分别为7.107和8.500。结论Ki67、CD34、D2-40、PD-L1的表达可预测PTC颈部淋巴结转移,同时为PTC生物学行为评估、预后判断及治疗方案的制定提供指导作用。
Objective To explore the value of Ki67,CD34,D2-40,PD-L1 and BRAF V600 E gene mutation in predicting cervical lymph node metastasis of papillary thyroid carcinoma(PTC).Methods One hundred and forty-seven PTC were selected to detect the expression of Ki67,CD34,D2-40 and PD-L1 by immunohistochemical technique and BRAF V600 E mutation by Real-time PCR.The expression differences of various molecular markers in PTC lesions with cervical lymph node metastasis and without cervical lymph node metastasis were compared,and the parameters were analyzed by multivariate regression analysis.Results Cervical lymph node metastases in PTC was associated with Ki67,CD34,D2-40 and PD-L1(P<0.05).BRAF V600 E was not associated with cervical lymph node metastasis of PTC(P>0.05).On multivariate analysis,Ki67 and CD34 were independent factors for predicting cervical lymph node metastasis of PTC(P<0.05,OR=7.107 and 8.500,respectively).Conclusion The expression of Ki67,CD34,D2-40 and PD-L1 could predict cervical lymph node metastasis of PTC,and guide the assessment of biological behavior of PTC,prognosis judgment and treatment plan.
作者
陈琪
张聪
崔璐莹
于波
李宜恩
赵书茵
马广容
吴长君
CHEN Qi;ZHANG Cong;CUI Lu-ying;YU Bo;LI Yi-en;ZHAO Shu-yin;MA Guang-rong;WU Chang-jun(Department of Ultrasound,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)
出处
《哈尔滨医科大学学报》
CAS
2019年第5期501-505,共5页
Journal of Harbin Medical University
基金
国家自然科学基金面上项目(81671697)
哈尔滨市科技创新人才研究专项资金项目(2011RFXYS047)
