摘要
该研究旨在从高温大曲中筛选得到适应酿造环境的高产淀粉酶土著菌株,用于大曲强化,以期提高白酒生产的淀粉利用率及提升白酒品质。采用淀粉水解圈法从高温大曲中分离得到1株产淀粉酶的菌株JM,经显微形态、生理生化特性及转录间隔区序列测序和系统进化树分析鉴定为扣囊复膜酵母(Saccharomycopsis fibuligera)。对该酵母进行产酶、耐受性及产香研究,结果表明,菌株JM液体培养10~12 h时产酶最高,为1452 U/mL;能耐受20%(体积分数)的酒精浓度、30%的糖度和17%的NaCl,在pH 3.0、49℃条件下均能较好生长。分别以小麦、大米、麸皮3种不同原料进行液态发酵试验,在小麦培养基中发酵产酒精达9.3%(体积分数),且能产生苯乙醇、乙酸乙酯、辛酸乙酯等37种呈香呈味物质,大米培养基中发酵产生29种香味物质,其中苯乙醇含量在3种原料发酵液中均为最高。该菌产淀粉酶及产酒、产香能力都比较强,在白酒酿造中有很好的应用潜质。
The aim of this work was to obtain the indigenous strain in traditional Chinese Spirit fermentation starter,“Daqu”,with high amylase compacity,which could be used for Daqu fortification to improve the starch utilization rate and the quality of Baijiu.A strain of amylase-producing strain JM was isolated from high-temperature Daqu by starch hydrolyzation method.It was identified as Saccharomycopsis fibuligera by microscopic morphology,physiological and biochemical characteristics,transcribed sequencing of spacer sequence and phylogenetic tree analysis.The production capacity of amylase,tolerability and aroma-production capacity of the yeast were studied.The result showed that 1452 U/mL amylase activities could be obtained when it was cultured for 10-12 h.The strain could tolerate 20%ethanol and grow well at pH 3 and 49℃.The liquid fermentation experiments were carried out with wheat,rice and bran as raw materials respectively.The results indicated that 9.3%of alcohol and 37 kinds of flavoring components such as phenylethyl alcohol,ethyl acetate and ethyl octanoate were produced in the wheat medium.In the rice medium,29 kinds of aroma substances,among which the phenylethyl alcohol content was the highest.The strain has strong ability of producing amylase,alcohol and aroma which present a good application potential in Baijiu production.
作者
周阳子
边名鸿
刘文艺
蒋佳静
许文静
ZHOU Yangzi;BIAN Minghong;LIU Wenyi;JIANG Jiajing;XU Wenjing(College of Mechanical Engineering,Sichuan University of Science&Engineering,Institute of Bioengineering,Zigong 643000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第2期79-84,共6页
Food and Fermentation Industries
基金
四川理工学院研究生创新基金(Y2018068)
关键词
高温大曲
酵母
淀粉酶
分离鉴定
耐受性
发酵
high-temperature Daqu
yeast
amylase
isolation and identification
tolerance
fermentation
