摘要
目的探讨中药茉莉联合远程缺血后适应(RIPOC)对急性心肌梗死大鼠心肌是否有协同保护作用。方法清洁级SD大鼠46只,复制大鼠急性心肌梗死模型,随机分为四组,即模型组(10只)、中药茉莉组(12只)、RIPOC组(12只)和联合组(中药茉莉+RIPOC)(12只);另取10只健康SD大鼠作为假手术组。通过超声心动检测心功能指标[左心室射血分数(LVEF)、左心室收缩末期容积(LVESV)和左心室舒张末期容积(LVEDV)],称重计算心脏重量指数(HWI)、左心室质量指数(LVMI),并检测心肌组织中丙二醛(MDA)和超氧化物歧化酶(SOD)含量。结果中药茉莉组、RIPOC组和联合组的LVEF、SOD含量均高于模型组,LVESV、LVEDV、HWI、LVMI及MDA含量均低于模型组,差异有统计学意义(P<0.05或P<0.01)。模型组的LVEF低于假手术组,HWI和LVMI均高于假手术组,差异有统计学意义(P<0.01)。模型组的MDA含量高于假手术组,SOD含量低于假手术组,差异有统计学意义(P<0.01)。结论中药茉莉和RIPOC在保护心肌梗死大鼠的心功能和心室重构中存在协同作用,值得推广。
Objective To explore the synergistic protective effect of Chinese medicine Jasmine combined with remote ischemic postconditioning(RIPOC)on myocardium in rats with acute myocardial infarction.Methods Forty-six clean SD rats were used to replicate the model of acute myocardial infarction and randomly divided into four groups,namely the model group(n=10),the Chinese medicine Jasmine group(n=12),the RIPOC group(n=12)and the combined group(Chinese medicine Jasmine+RIPOC)(n=12).Another 10 healthy SD rats were selected as the sham operation group.Cardiac function indicators(left ventricular ejection fraction[LVEF],left ventricular end-systolic volume[LVESV],and left ventricular end-diastolic volume[LVEDV])were measured by echocardiography,cardiac weight index(HWI)and left ventricular mass index(LVMI)were calculated by weighing,and the contents of malondialdehyde(MDA)and superoxide dismutase(SOD)in myocardial tissue were detected.Results The LVEF and content of SOD in the Chinese medicine Jasmine group,RIPOC group and combined group were higher than those in the model group,the LVESV,LVEDV,HWI,LVMI and content of MDA were lower than those in the model group,and the differences were statistically significant(P<0.05 or P<0.01).The LVEF of the model group was lower than that of the sham operation group,HWI and LVMI were higher than those of the sham operation group,and the differences were statistically significant(P<0.01).The MDA content in the model group was higher than that in the sham operation group,the SOD content was lower than that in the sham operation group,and the differences were statistically significant(P<0.01).Conclusion Chinese medicine Jasmine combined with RIPOC has a synergistic effect on the protection of cardiac function and ventricular remodeling in rats with acute myocardial infarction,which is worth promoting.
作者
栾海蓉
代海兵
王菡
吴红
郑学芝
LUAN Hai-rong;DAI Hai-bing;WANG Han;WU Hong;ZHENG Xue-zhi(Department of Function,Mudanjiang Medical University,Heilongjiang Province,Mudanjiang157011,China;School of Clinical Medicine,Mudanjiang Medical University,Heilongjiang Province,Mudanjiang157011,China;Department of Physiology,Mudanjiang Medical University,Heilongjiang Province,Mudanjiang157011,China)
出处
《中国当代医药》
2020年第3期4-7,共4页
China Modern Medicine
基金
黑龙江省省属高等学校基本科研业务费科研项目（2017-KYYWFMY-0662）
关键词
心肌梗死模型
中药茉莉
远程缺血后适应
联合干预
丙二醛
超氧化物歧化酶
左心室射血分数
左心室质量指数
Acute myocardial infarction Model
Chinese medicine Jasmine
Remote ischemic postconditioning
Combined intervention
Malondialdehyde
Superoxide dismutase
Left ventricular ejection fraction
Left ventricular mass index
