摘要
目的:观察胃促生长素(Ghrelin)对大鼠胃排空及食管下括约肌(LES)压力的影响,同时测定血浆中胃动素和P物质水平的变化,探讨Ghrelin对胃排空及LES压力的影响及相关机制。方法:Wistar大鼠接受不同剂量Ghrelin(0.5、1.0、1.5、2.0、2.5 nmol/kg)腹腔注射后,测定胃排空率;Wistar大鼠接受1.5 nmol/kg Ghrelin腹腔注射后进行LES压力测定,放射免疫法测定血浆中胃动素及P物质浓度。结果:腹腔注射Ghrelin后大鼠胃排空率为38.24%±7.15%,与对照组(27.03%±2.91%)比明显加快(P<0.01),同时该效应与腹腔注射Ghrelin的量呈量效关系,腹腔注射Ghrelin后大鼠LES压力[(19.57±3.26)mmHg]与对照组[(13.78±2.29)mmHg]比明显增加(P<0.05),提示Ghrelin能增加大鼠LES压力;腹腔注射Ghrelin后大鼠血浆胃动素浓度[(217.28±25.38)ng/L]与对照组[(190.02±18.18)ng/L]比明显增加(P<0.05),提示胃动素可能参与Ghrelin对大鼠食管下括约肌压力的调节。结论:中、高剂量Ghrelin腹腔注射,能明显加快健康大鼠的胃排空效应,Ghrelin能明显增加LES压力,胃动素可能参与Ghrelin对大鼠LES压力的调节。
Objective:To investigate the effects of Ghrelin on gastric emptying and lower esophageal sphincter pressure(LESP),expressions of Motilin and Substance P(SP)in plasma,and to explore the mechanism of Ghrelin on gastric emptying and LESP.Methods:After Wistar rats were intraperitioneally injected with different doses of Ghrelin(0.5,1.0,1.5,2.0,2.5 nmol/kg),gastric emptying rate was measured;after intraperitoneal injecion of 1.5 nmol/kg,LESP was measured,and plasma Motilin and SP concentrations were determined by radioimmunoassay.Results:After intraperitoneal injection of Ghrelin,gastric emptying rate was significantly faster than controls[(38.24±7.15)%vs.(27.03±2.91)%],and the accelerated gastric emptying had a significant dose-response relationship with Ghrelin.After intraperitoneal injection of Ghrelin,LESP[(19.57±3.26)mmHg]in rats was significantly increased compared with the control group[(13.78±2.29)mmHg](P<0.05),suggested that Ghrelin could increase the LESP in rats.After intraperitoneal injection of Ghrelin,plasma Motilin concentration[(217.28±25.38)ng/L]in rats was significantly increased compared with the control group[(190.02±18.18)ng/L](P<0.05),suggesting that Motilin may be involved in the LESP regulation in rats.Conclusion:Intraperitoneal injection of Ghrelin can accelerate gastric emptying in rats,Ghrelin may increase the pressure of the lower esophageal sphincter in rats,and Motilin may be involved in the LESP regulation.
作者
曹曙光
吴昊
林李淼
CAO Shuguang;WU Hao;LIN Limiao(Department of Gastroenterology,the Second Affiliated Hospital&Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou 325027,China)
出处
《温州医科大学学报》
CAS
2020年第2期158-161,共4页
Journal of Wenzhou Medical University
基金
温州市科技计划项目（Y20150166）
关键词
促生长素
胃动素
胃排空
食管下括约肌压力
Ghrelin
Motilin
gastric emptying
lower esophageal sphincter pressure
