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石榴皮鞣质对前列腺癌细胞侵袭转移影响的研究 被引量:2

Effect of pomegranate tannin on invasion and metastasis of prostate cancer cells
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摘要 目的探讨石榴皮鞣质对人前列腺癌PC-3细胞侵袭转移的影响。方法以人前列腺癌PC-3细胞为靶细胞,通过划痕愈合实验检测石榴皮鞣质对体外培养的PC-3细胞运动能力的影响;采用Transwell小室实验(无基质胶)检测石榴皮鞣质对PC-3细胞运动能力的影响,采用Transwell小室实验(有基质胶)检测石榴皮鞣质对PC-3细胞侵袭能力的影响;采用裸鼠肺转移模型检测石榴皮鞣质对PC-3细胞体内侵袭能力的影响。结果划痕愈合实验结果显示,石榴皮鞣质能够抑制PC-3细胞的运动性,随着石榴皮鞣质浓度增加,其抑制PC-3细胞运动能力也逐渐增强,呈明显的浓度依赖性,与空白对照组比较差异有统计学意义(P<0.05);Transwell小室实验(无基质胶)显示,石榴皮鞣质能够抑制PC-3细胞运动;与空白对照组相比,随着石榴皮鞣质浓度增加,其降低PC-3细胞运动性也逐渐增强(P<0.05);Transwell小室(有基质胶)侵袭实验显示,与空白对照组相比,含有不同浓度石榴皮鞣质的实验组中,PC-3细胞穿膜细胞数即抑制其侵袭性得到有效抑制,与空白对照组相比,随着药物浓度的增加,其抑制侵袭性能力也逐渐增强(P<0.05);此外,裸鼠肺转移模型结果显示,石榴皮鞣质能够抑制PC-3细胞的侵袭性;与空白对照组相比,随着石榴皮鞣质浓度增加,PC-3细胞形成的肺转移瘤减少(P<0.05)。结论石榴皮鞣质对人前列腺癌PC-3细胞侵袭转移具有抑制作用,能抑制前列腺癌的侵袭转移。 Objective To investigate the effect of pomegranate tannin on invasion and metastasis of human prostate cancer PC-3 cells.Methods Using human prostate cancer PC-3 cells as target cells,the effect of pomegranate tannin on the migration ability of PC-3 cells in vitro was detected by scratch healing test;the effect of pomegranate tannin on the migration ability of PC-3 cells was detected by Transwell chamber test(without matrix gum);the effect of pomegranate tannin on the invasion ability of PC-3 cells was detected by Transwell chamber test(with matrix gum).The effect of pomegranate tannin on invasion ability of PC-3 cells in vivo was detected by nude mice lung metastasis model.Results Scratch healing experiment showed that pomegranate tannin could inhibit the movement of PC-3 cells.With the increase of pomegranate tannin concentration,pomegranate tannin inhibited the movement of PC-3 cells in a concentration-dependent manner,which was significantly different from that of control group(P<0.05).Transwell experiment(without matrix gel)showed that pomegranate tannin could inhibit the movement of PC-3 cells;compared with control group,pomegranate tannin inhibited the movement of PC-3 cells in a concentration-dependent manner(P<0.05).Increasing the concentration of pomegranate tannin could decrease the motility of PC-3 cells(P<0.05).Transwell(with matrix gel)invasion experiment showed that the number of PC-3 cells which penetrated the membrane was inhibited in the experimental groups with different concentrations of pomegranate tannin,and the invasion of PC-3 cells was inhibited.Compared with control group,the ability of inhibiting invasion of PC-3 cells increased with the increase of drug concentration(P<0.05).In addition,the results of lung metastasis model in nude mice showed that pomegranate tannin could inhibit the invasion of PC-3 cells.Compared with control group,the number of lung metastases formed by PC-3 cells decreased with the increase of pomegranate tannin concentration(P<0.05).Conclusion The pomegranate tannin can inhibit the invasion and metastasis of human prostate cancer PC-3 cells and the prostate cancer.
作者 潘正波 张海涛 蔡海荣 胡恩平 刘晟 PAN Zheng-bo;ZHANG Hai-tao;CAI Hai-rong;HU En-ping;LIU Sheng(Department of Urology,Taizhou Municipal Hospital,Taizhou 318000,China)
出处 《实用药物与临床》 CAS 2020年第2期117-121,共5页 Practical Pharmacy and Clinical Remedies
关键词 石榴皮鞣质 前列腺癌 侵袭 转移 Pomegranate tannin Prostate cancer Invasion Metastasis
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  • 1Rui Chen,Shancheng Ren,无,Ming Kwong Yiu,Ng Chi Fai,Wai Sam Cheng,Lap Hong Ian,Seiji Naito,Tadashi Matsuda,Elijah Kehinde,Ali Kural,Jason Yichun Chiu,Rainy Umbas,Qiang Wei,Xiaolei Shi,Liqun Zhou,Jian Huang,Yiran Huang,Liping Xie,Lulin Ma,Changjun Yin,Danfeng Xu,Kexin Xu,Zhangqun Ye,Chunxiao Liu,Dingwei Ye,Xin Gao,Qiang Fu,Jianquan Hou,Jianlin Yuan,Dalin He,Tiejun Pan,Qiang Ding,Fengshuo Jin,Benkang Shi,Gongxian Wang,Xiuheng Liu,Dongwen Wang,Zhoujun Shen,Xiangbo Kong,Wanhai Xu,Yaoliang Deng,Haibo Xia,Alexa NCohen,Xu Gao,Chuanliang Xu,Yinghao Sun.Prostate cancer in Asia:A collaborative report[J].Asian Journal of Urology,2014,1(1):15-29. 被引量:38
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