摘要
背景:有研究表明枸杞多糖具有抗老化、保护神经、抗疲劳、控制血糖、抗氧化及抗肿瘤等作用,其对于膝关节的骨性关节炎可能具有一定的保护作用,但目前还鲜有学者报道,CD151和基质金属蛋白酶3是评价膝关节骨性关节炎常用的因子。目的:观察枸杞多糖对CD151和基质金属蛋白酶3在兔骨性关节炎中表达的影响。方法:健康6月龄大耳白兔64只随机分等为4组:空白组、模型组、枸杞多糖组及生理盐水组。除了空白组外,其他3组均采用Hulth法复制膝骨关节炎模型兔,枸杞多糖组及生理盐水组以常规剂量枸杞多糖及生理盐水灌胃饲养4周。造模后12周取各组患侧关节软骨组织,应用苏木精-伊红染色观察各组关节软骨形态学变化,应用免疫组织化学染色及Western blot法观察CD151和基质金属蛋白酶3在各组关节软骨中的表达水平及空间分布。实验于2014年1月经宁夏人民医院动物伦理委员会批准(批准号:2014-30817)。结果与结论:①免疫组织化学染色和Western blot检测显示,枸杞多糖组关节软骨组织中CD151和基质金属蛋白酶3吸光度值及蛋白表达均低于生理盐水组与模型组(P<0.05);②上述结果证实,在骨性关节炎关节软骨组织中CD151和基质金属蛋白酶3表达增高,枸杞多糖可抑制骨性关节炎中CD151和基质金属蛋白酶3的高表达,从而减缓骨性关节炎的发生。
BACKGROUND:Studies have shown that Lycium barbarum polysaccharide(LBP)has the functions of anti-aging,nerve protection,anti-fatigue,blood sugar control,anti-oxidation,and anti-tumor.It may have some protective effects against osteoarthritis of the knee,but have been rarely reported.CD151 and matrix metalloproteinase 3(MMP-3)are two common cytokines for assessing knee osteoarthritis.OBJECTIVE:To observe the effect of LBP on the expression of CD151 and MMP-3 in rabbit osteoarthritis.METHODS:Sixty-four healthy 6-month-old white rabbits were randomly divided into four groups:blank group,model group,LBP group and normal saline group.Animal models of knee osteoarthritis were made using Hulth method in the rabbits except those in the blank group.The rats in the LBP and normal saline groups were fed with normal dose of LBP and normal saline for 4 weeks,and then the articular cartilage tissues were taken from the affected side at 12 weeks after modeling.The morphological changes of the articular cartilage were observed by hematoxylin-eosin staining.The expression levels and spatial distribution of CD151 and MMP-3 in articular cartilage was observed by immunohistochemical staining and western blot.Ethic approval was given by the People’s Hospital of Ningxia Hui Autonomous Region(approval No.2014-30817).RESULTS AND CONCLUSION:immunohistochemistry staining and western blot results showed that the absorbance values and protein expression of MMP-3 and CD-151 were significantly lower in the LBP group than the normal saline and model groups(P<0.05).Therefore,the expression of CD151 and MMP-3 in the articular cartilage of osteoarthritis was increased,and LBP could inhibit the expression of CD151 and MMP-3 in osteoarthritis,so as to slow down the occurrence of osteoarthritis.
作者
赵飞
丁冬
巩凡
黄永禄
姚占川
李晓亮
罗小军
岳建明
Zhao Fei;Ding Dong;Gong Fan;Huang Yonglu;Yao Zhanchuan;Li Xiaoliang;Luo Xiaojun;Yue Jianming(Department of Hand&Foot&Reconstructive Microsurgery,Ningxia Medical University Affiliated Autonomous Region People's Hospital,Yinchuan 750001,Ningxia Hui Autonomous Region,China)
出处
《中国组织工程研究》
CAS
北大核心
2020年第17期2648-2653,共6页
Chinese Journal of Tissue Engineering Research
基金
卫生部手功能重建重点实验室&上海市周围神经显微外科重点实验室开放课题(17DZ2270500)：项目负责人：赵飞
中央高校基本科研业务费专项资金项目(31920160099)：项目负责人：赵飞
宁夏科技支撑对外科技合作项目(2015第14号)：项目负责人：赵飞
宁夏自然科学基金项目(NZ16177)，项目负责人：赵飞
宁夏自然科学基金项目(NZ16182)，项目负责人：巩凡
宁夏自然科学基金项目(NZ13182)，项目负责人：岳建明~~
