摘要
采用平板分离技术从烟草根际土壤中分离到一个菌株,编号为XXJ15,并对该菌株的几种活性功能进行了评估。结果表明,XXJ15在魔芋粉液体鉴别培养基经过48 h发酵后,发酵液中的β-甘露聚糖酶活性达到4.6 U/mL;在解磷液体鉴别培养基中培养72 h后,培养基上清液中游离态磷含量达到102μg/mL;XXJ15不具备解钾活性。因此,菌株XXJ15可作为后期诱变育种、烟草专用菌肥和土壤保育剂开发等研究的备用菌株。
A strain named XXJ15 was isolated from tobacco rhizosphere soil by plate separation,and the activity function of the strain were evaluated.The results showed that after 48 h fermentation in the liquid identification medium of konjac powder,the activity of β-mannanase in the fermentation broth reached 4.6 U/mL.After culture for 72 h in the phosphorus solution identification medium,the free phosphorus content in the supernatant of the medium reached 102 μg/mL.XXJ15 didn’t have potassium activity.Therefore strain XXJ15 can be used as a backup strain for later mutagenesis breeding,tobacco special bacterial fertilizer and soil conservation agent development.
作者
许晓敬
唐培培
禹宝成
郭传滨
杨立均
孙会忠
XU Xiao-jing;TANG Pei-pei;YU Bao-Cheng;GUO Chuan-bin;YANGLi-jun;SUN Hui-zhong(Zhumadian Branch of Henan Provincial Tobacco Company,Zhumadian Henan 463000;Wuhan Cigarette Factory)
出处
《现代农业科技》
2020年第2期174-174,181,共2页
Modern Agricultural Science and Technology
基金
河南省烟草公司驻马店市公司项目(植烟土壤的微生物定向调控保育技术研究)
河南省高等学校重点科研项目(16A180003)
关键词
植烟土壤
酶活性
解磷菌
分离
活性评估
tobacco-growing soil
enzyme activity
phosphate-solubilizing bacteria
isolation
activity evaluation
