摘要
目的:探讨肺结核患者外周血单个核细胞(PBMCs)中核转录因子kappa B1(NFκB1)与长链非编码RNA PACER(LncRNA-PACER)的表达关系。方法:收集本院确诊的肺结核患者40例(肺结核组)及同期健康体检者40例(对照组),采用酶联免疫吸附(ELISA)法检测血清中肿瘤坏死因子-α(TNF-α)、白介素(IL)-6和IL-8水平;实时荧光定量PCR法检测PBMCs中LncRNA-PACER、NFκB1 mRNA表达;Western blot检测PBMCs中NFκB1、COX2蛋白表达;Pearson法分析肺结核患者PBMCs中LncRNA-PACER、NFκB1表达的相关性;分析肺结核患者PBMCs中LncRNA-PACER、NFκB1表达与临床病理特征的关系。结果:与对照组相比,肺结核组血清中TNF-α、IL-6和IL-8表达均显著升高(P<0.05),PBMCs中LncRNA-PACER、NFκB1 mRNA及蛋白、COX2蛋白表达均显著升高(P<0.05)。PBMCs中LncRNA-PACER、NFκB1蛋白表达水平与患者肺部病灶数量、肺部空洞有关(P<0.05),肺结核患者PBMCs中LncRNA-PACER表达与NFκB1 mRNA表达呈正相关(r=0.873,P<0.05)。结论:肺结核患者PBMCs中NFκB1、LncRNA-PACER表达显著升高,二者呈正相关,均与肺结核发生发展密切相关。
Objective: To investigate the expression relationship between nuclear transcription factor kappa B 1(NFκB1) and long non-coding RNA PACER(LncRNA-PACER) in peripheral blood mononuclear cells(PBMCs) of patients with pulmonary tuberculosis. Methods: From February 2018 to March 2019, 40 patients with pulmonary tuberculosis(tuberculosis group) and 40 healthy persons(control group) were collected, the levels of TNF-α, IL-6 and IL-8 in serum were detected by enzyme-linked immunosorbent assay(ELISA);the expressions of LncRNA-PACER and NFκB1 mRNAs in PBMCs were detected by real-time fluorescence quantitative PCR;Western blot was used to detect the expressions of NFκB1 and COX 2 in PBMCs;Pearson method was used to analyze the expressions of LncRNA-PACER and NFκB1 in PBMCs of patients with pulmonary tuberculosis;and the expressions of LncRNA-PACER and NFκB1 in PBMCs of patients with pulmonary tuberculosis were analyzed. Results: Compared with the control group, the expressions of TNF-α, IL-6 and IL-8 in the serum of patients with pulmonary tuberculosis was significantly increased(P<0.05), and the expressions of LncRNA-PACER, NFκB1 mRNAs, proteins and COX-2 protein in PBMCs were significantly increased(P<0.05). The expressions of LncRNA-PACER and NFκB1 proteins in PBMCs were related to the number of pulmonary lesions and pulmonary cavity(P<0.05), and there was a positive correlation between the expression of LncRNA-PACER and the expression of NFκB1 mRNA in PBMCs of patients with pulmonary tuberculosis(r=0.873, P<0.05). Conclusions: The expressions of NFκB1 and LncRNA-PACER in PBMCs of patients with pulmonary tuberculosis are significantly increased, they are positively correlated and both of them are related to the occurrence and development of pulmonary tuberculosis.
作者
黄东轩
何朝文
廖毅力
彭剑锋
杨帆
曹亚辉
黄冬生
HUANG Dong-Xuan;HE Zhao-Wen;LIAO Yi-Li;PENG Jian-Feng;YANG Fan;CAO Ya-Hui;HUANG Dong-Sheng(Department of respiratory and critical care medicine,Shenzhen longhua district central hospital.ShenZhen Longhua 518110;Department of obstetrics,Shenzhen Maternity&Child Healthcare Hospital.ShenZhen Futian 518028)
出处
《海南医学院学报》
CAS
2020年第4期285-289,共5页
Journal of Hainan Medical University
基金
深圳市科技计划(JCYJ20180306172419505)~~
