摘要
应用滞育技术是延长赤眼蜂货架期的可行性措施之一。为实现工厂化生产稻螟赤眼蜂滞育蜂,本研究以在3种光周期(16 L∶8 D、12 L∶12 D和8 L∶16 D)下繁育的稻螟赤眼蜂种群为材料,考察了诱导始期(寄生后发育12 h、24 h、36 h、48 h、60 h和72 h)、诱导温度(10℃和13℃)和诱导历期(14 d、21 d、28 d和35 d)对稻螟赤眼蜂滞育的影响。研究结果表明短光照下繁育的种群更容易进入滞育状态;寄生后发育24 h和12 h为最敏感的诱导始期,随着诱导始期虫龄的增长,滞育效果也随之下降;诱导温度对滞育也有显著影响,其中8 L∶16 D的种群在13℃下诱导的滞育率要显著高于10℃,但其他两个种群在10℃下诱导的滞育率要显著高于13℃;诱导历期的增加可以显著提高稻螟赤眼蜂的滞育率。供试稻螟赤眼蜂种群的较适宜滞育条件为8 L∶16 D下繁育的赤眼蜂种群寄生后发育12 h于13℃下诱导滞育28 d或35 d,以及寄生后发育24 h于10℃或13℃下诱导滞育35 d,滞育率可超过95%。
Application of diapause technology is one of the feasible measures to extend the shelf-life of Trichogramma.In order to realize industrialization of Trichogramma japonicum,three T.japonicum populations,which were reared under three different photoperiod(16L∶8D,12 L∶12 D and 8 L∶16 D),were used in the present study.The effect of inducing initial stage(12 h,24 h,36 h,48 h,60 and 72 h),inducing temperature(10℃and 13℃)and inducing period(14 d,21 d,28 d and 35 d)on diapause rate of T.japonicum were investigated.The results showed that the diapause rate of T.japonicum population that reared under photoperiod 8 L∶16 D was higher than the others.24 h and 12 h are the most sensitive inducing initial stage,and the diapause rate was decreased with the increasing the age of T.japonicum at inducing initial stage.The inducing temperature also had a significant effect on diapause rate.The diapause rate of the 8 L∶16 D population induced at 13℃was significantly higher than those induced at 10℃.However,the diapause rate of the other two populations induced at 10℃was significantly higher than 13℃.And the increase of inducing period could significantly improved the diapause rate of T.japonicum.The optimal conditions for diapause induction of the tested T.japonicum populations were 12 hold of the 8 L∶16 D population induced at 13℃after 28 d or 35 d,and 24 hold of the 8 L∶16 D population induced at 10℃or 13℃after 35 d.The diapause rate of T.japonicum were above 95%under these induced conditions.
作者
时亚东
田俊策
王国荣
鲁艳辉
郑许松
吕仲贤
SHI Ya-Dong;TIAN Jun-Ce;WANG Guo-Rong;LU Yan-Hui;ZHENG Xu-Song;L Zhong-Xian(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,Zhejiang Province,China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Science,Hangzhou 310021,China;Xiaoshan Rural Agriculture Bureau,Hangzhou 311203,China)
出处
《环境昆虫学报》
CSCD
北大核心
2020年第1期22-28,共7页
Journal of Environmental Entomology
基金
国家重点研发计划(2016YFD0200800)
现代农业产业技术体系(CARS-01-17)
浙江省农科院2019年度青年人才培养项目
关键词
赤眼蜂
滞育
光周期
诱导始期
诱导历期
Trichogramma
diapause
photoperiod
inducing initial stage
inducing period
