摘要
目的研究复方降脂颗粒对2型糖尿病(T2DM)大鼠的降血糖作用及其可能的作用机制。方法高糖高脂饲料喂养联合腹腔注射小剂量链脲佐菌素(STZ)诱导T2DM模型。造模成功的20只大鼠随机分成两组,分别是模型组和复方降脂颗粒组。复方降脂颗粒组灌胃600mg/kg复方降脂颗粒溶液,正常组和模型组分别给予等量0.9%NaCl注射液。连续给药8周后,用试剂盒检测各组大鼠血清空腹血糖(FBG)和空腹胰岛素(FINS)水平,计算胰岛素敏感指数(ISI),并对各组大鼠胰腺组织进行苏木精-伊红(HE)染色以观察病理形态的改变。qRT-PCR和Western blot法检测大鼠胰腺组织中磷脂酰肌醇-3激酶(PI 3K)、磷酸肌醇依赖性激酶-1(PDK-1)、磷酸化蛋白激酶B(pAKT)mRNA和蛋白表达水平的变化。结果复方降脂颗粒干预可显著降低T2DM大鼠血清中FBG、FINS的水平,同时上调ISI值(P<0.05)。复方降脂颗粒组的大鼠胰腺组织损伤程度明显减轻,胰岛数目增多的同时伴随水肿变形程度减轻。复方降脂颗粒干预后,大鼠胰腺组中的PI 3K、PDK-1、pAKT mRNA和蛋白的表达均上调,显著高于模型组。结论复方降脂颗粒能明显降低2型糖尿病大鼠血糖水平,改善胰岛素抵抗和胰腺损伤的情况,其机制可能与PI 3K/AKT通路的激活有关。
Objective To investigate the hypoglycemic effect and mechanism of Fufang Jiangzhi Granule on type 2 diabetic rats.Methods Type 2 diabetes mellitus(T2DM)rat model was induced by high-sugar and high-fat diet combined with low-dose streptozotocin(STZ).The rats were divided into the normal group,model group and Fufang Jiangzhi Granule group,10 rats in each group.The Fufang Jiangzhi Granule group was treated with 600 mg/kg Fufang Jiangzhi Granule.The normal group and the model group were treated with saline at equivalent volume.After treatment 8 weeks,the fasting blood glucose(FBG)and fasting insulin(FINS)were measured by kits and the insulin sensitivity index(ISI).The pancreas were removed to assess the pathological by HE staining.The mRNA and protein expression level of phosphatidulinositol-3-kinase(PI 3K),phosphoinositide dependent kinase-1(PDK-1)and phosphorylated protein kinase B(pAKT)in pancreas were detected by qRT-PCR and Western blot.Results Compared with those in normal group,Fufang Jiangzhi Granule could effectively reduced the FBG、FINS levels,increased ISI levels(P<0.05).Fufang Jiangzhi Granule could significantly decrease the severities of pancreas tissue injury in test groups,while the numbers of islet increased,and the edema and deformation of cells were relieved.The mRNA and protein expression of PI 3K,PDK-1 and pAKT increased obviously,which were significant higher than those in model groups(P<0.05).Conclusion Fufang Jiangzhi Granule has significant hypoglycemic effect on T2DM rats with the insulin resistance improved.Its mechanism may be associated with the activation of the PI 3K/AKT signaling pathway.
作者
王洪霞
王贤娴
Wang Hongxia;Wang Xianxian(Department of Geriatrics,Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region,Xinjiang 830000,China)
出处
《医学研究杂志》
2020年第2期105-109,共5页
Journal of Medical Research
基金
新疆维吾尔自治区自然科学基金资助项目(2017D01C329)
新疆医科大学附属中医医院院级基金资助项目(ZYY201611)。
关键词
复方降脂颗粒
2型糖尿病
血糖
胰岛素
胰腺
Fufang Jiangzhi Granule
Type 2 diabetes mellitus
Blood glucose
Insulin
Pancreas
