摘要
在前期研究中,从二倍体马铃薯中鉴定了1个短花药突变体sa1,是培育不育系的良好材料。在本研究中,构建了sa1的BC1F2分离群体,通过混池测序的方法发现该性状由1个单基因控制,位于6号染色体0~33.9 Mb的区间。为了精细定位sa1,进一步扩大遗传群体,通过对重组单株进行基因型和表型分析,最终将sa1定位到1.4 Mb的区间。根据马铃薯参考基因组的注释信息,该区域包含40个基因,并通过转录组测序对候选基因进行了预测。
In a previous study,we identified a short anther mutant sal,which is a potential material for developing male sterile lines.In this study,we constructed a BC1 F2 segregating population of sal.By bulked segregant analysis(BSA-seq),we found this trait was controlled by a single gene which located in a 0-33.9 Mb interval on chromosome No.6.To further map the sal gene,we used more BC1 F2 progeny to screen the recombinants.Based on the genotype and phenotype of recombinants,the sal gene was narrowed to a 1.4 Mb interval.According to the annotation information of the potato reference genome,this region contains 40 genes.In addition,we predicted the candidate genes using the transcriptome data from normal and short anthers.
作者
王培
唐蝶
李玉梅
杨中敏
祝光涛
黄三文
张春芝
WANG Pei;TANG Die;LI Yumei;YANG Zhongmin;ZHU Guangtao;HUANG Sanwen;ZHANG Chunzhi(The CAAS-YNNU Joint Academy of Potato Sciences,Yunnan Normal University,Kunming 650500,China;Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen Guangdong 518120,China;Faculty of Information Engineering and Automation Kunming University of Science and Technology,Kunming 650500,China;College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2019年第12期2429-2436,共8页
Acta Horticulturae Sinica
基金
云南省科技厅高端人才引进计划项目（2013HA025）
中国农业科学院科技创新工程项目
